fa بررسی فراوانی سروتایپ‌های انتروپاتوژن دارای ژن‌ pap G در اشریشیاکلی جداسازی شده از ادرار و خون بیماران بستری در بیمارستان‌های شهرستان خرم‌آباد کارآزمایی بالینی Clinical Trials <pre> <span style="font-family:times new roman;"><strong><em>Background and Objective</em></strong><em>:</em> <em>Escherichia coli</em> (<em>E-coli</em>) is a normal gram negative organism that inhabit the human and animal intestinal tract. Pap G gene has an important role in creating P -fimbriae tip and in producing septicemia and urinary tract infection. The aim of this study was to investigate the presence of pap G gene in <em>EPEC</em> strains isolated from urine and blood. </span></pre> <pre> <span style="font-family:times new roman;"><strong><em>Materials and Methods:</em></strong> From March to October 2013, 90 colonies of urine and blood samples suspected to <em>Escherichia coli</em> were collected. The identity of isolates was determined by standard biochemical tests. Their antibiotic resistance pattern was detected by susceptibility disk diffusion method (Global standard CLSI) and the frequency of pap G gene was examined by Multiplex PCR and pap G positive isolates were serotyped against O antigen. </span></pre> <pre> <span style="font-family:times new roman;"><strong><em>Results:</em></strong> From 90 suspected colonies of urine and blood, 72 (80%) cases found to be E- coli. Out of 72 cases, 65 (90.27%) cases were isolated from urine and 7 (9.73%) cases isolated from blood. 30 out of 72 samples were positive for pap G (II, III) gene. Five out of 30 (16.6%) cases were found to belong to O26, O128, O125, O114 and O55 EPEC strains. </span></pre> <p><span style="font-family:times new roman;"><strong><em>Conclusion: </em></strong>In this study, the pap G (II, III) gene was observed in the isolated enteropathogenic E<em>- coli</em> from the hospitalized patients&#39; urine and blood samples. Therefore, entropathogenic strains of <em>E-coli</em> can also cause urinary tract infections and septicemia similar to uropathogenic strains which having pap G gene and P- fimbriae tip.</span></p> <p></p><p></p> Keywords: Escherichia coli, Multiplex PCR, pap G, Antibiotic resistance, Urinary infection. 102 111 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-945&slc_lang=fa&sid=1 Afsaneh Shaban افسانه شعبان shabanafsaneh@yahoo.com 5200319475328460053768 5200319475328460053768 Yes Dept. of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, Iran دانشگاه آزاد اسلامی واحد تهران Jamileh Norouzi جمیله نوروزی 5200319475328460053769 5200319475328460053769 No Dept. of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, Iran دانشگاه آزاد اسلامی واحد تهران