Journal of Advances in Medical and Biomedical Research

en Co-culture-based three-dimensional culture platforms to enhance the in vitro survival and proliferation of U266 myeloma cells Medical Biology Medical Biology مقاله پژوهشی Original Research Article Background and Objectives: It has been demonstrated that three-dimensional (3D) interactions of myeloma cells with other cells and components of the extracellular matrix (ECM) in the bone marrow (BM) microenvironment play critical roles in the disease progression. Therefore, in vitro fabrication of interactions would advance the fields of disease modeling and drug screening. In this regard, we tried to develop simple, cost-beneficial, and available 3D culture conditions for U266 cells using co-culture systems in order to be closer to their native microenvironment. Materials and Methods: U266 cells, BM mesenchymal stem cells (BM-MSCs) and human umbilical vein endothelial cells (HUVECs) were co-cultured in different matrix-based and matrix-free conditions, and thereafter, the survival and proliferation rates of U266 cells were evaluated and compared. Peripheral blood (PB) plasma was used to generate fibrin gels for matrix-based structures. Results: Co-cultured cells in the gel-free group could generate and assemble into 3D cell structures. Moreover, the viability and expansion fold of U266 cells in the gel-free group were significantly higher compared to the on-gel and inside-gel groups. Conclusion: Our simple gel-free 3D co-culture of U266 cells with BM-MSCs and HUVECs, which showed higher viability and proliferation rates of myeloma cells, can be used for further in vitro studies, including drug screening.   multiple myeloma, co-culture, three-dimensional cell culture, fibrin, proliferation 19 19 http://journal.zums.ac.ir/browse.php?a_code=A-10-7370-1&slc_lang=en&sid=1 Mohammadmahdi Khosravi mohammadmahdy2012@gmail.com 5200319475328460088999 5200319475328460088999 No Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Sadaf Vahdat s_vahdat@modares.ac.ir 5200319475328460089000 5200319475328460089000 Yes Applied Cell Sciences Division, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Saeid Abroun abroun@modares.ac.ir 5200319475328460089001 5200319475328460089001 No Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran