Journal of Advances in Medical and Biomedical Research

en Ameliorative Role of Resveratrol in Silver Nanoparticle–Induced Oxidative Stress and Testicular Alterations in Male Mice Pharmacology Pharmacology مقاله پژوهشی Original Research Article Background: Silver nanoparticles (AgNPs) are among the most commonly used silver derivatives, which, due to their potential reproductive toxicity, can be considered a potential hazard for fertility. Objective: The study aimed to evaluate the protective effect of resveratrol against AgNPs-induced testicular damage and oxidative stress in male mice model. Materials and Methods: In this experimental study, 24 adult male mice were randomly divided into four groups (n=6/group): control, AgNPs (200 mg/kg), resveratrol (20 mg/kg), and a combination of AgNPs and resveratrol. Treatments were administered via daily gavage for 35 days. Finaly, serum malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD), testosterone, testicular 8-OHdG levels, and histological changes included seminiferous tubule diameter, Leydig cell count, tissue structure, and spermatogenic status were analyzed between groups. Results: AgNPs significantly reduced serum testosterone levels and increased oxidative stress, as indicated by elevated SOD and 8-OHdG levels (P< 0.001). Resveratrol treatment significantly restored testosterone levels and reduced oxidative DNA damage, with values approaching those of the control group (P< 0.01). No significant changes were observed in TAC and MDA levels among the groups. AgNPs caused marked reductions in seminiferous tubule diameter, epithelial thickness, and counts of primary spermatocytes, spermatozoa, Sertoli, and Leydig cells (P<0.001). These adverse effects were significantly ameliorated by Resveratrol, particularly in the Resveratrol-only group (P<0.01). Conclusion: AgNPs induce oxidative stress-mediated testicular damage and hormonal disruption in male mice. While resveratrol exerts a protective role by mitigating some of these effects, its impact was limited and did not fully reverse AgNP-induced toxicity.   Silver nanoparticles, Resveratrol, Reproductive Toxicity, Testicular Damage, Mice.Mice. 20 20 http://journal.zums.ac.ir/browse.php?a_code=A-10-4479-2&slc_lang=en&sid=1 Mohammad amin behmanesh behmanesh.ma@yahoo.com 5200319475328460089002 5200319475328460089002 No 1. Department of Histology, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran sima janati sjanati@ymail.com 5200319475328460089003 5200319475328460089003 No 2. Department of Obstetrics and Gynecology, School of Medicine, Research and Clinical Center for Infertility, Dezful University of Medical Sciences, Dezful, Iran rezvan reyhani rezvan98reyhani@yahoo.com 5200319475328460089004 5200319475328460089004 No 3. Student research committee, Dezful University of medical science, Dezfu, Iran Seyedeh Mahsa poormoosavi m.poormosavi@ymail.com 5200319475328460089005 5200319475328460089005 Yes 4. Department of Histology, School of Medicine, Research and Clinical Center for Infertility Dezful University of Medical Sciences, Dezful, Iran