en Life Science Life Science مقاله پژوهشی Original Research Article <span style="font-size:12pt"><span style="background:white"><span style="line-height:18.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="color:black">Background and Objective:</span></b> <span style="color:black">Melanoma is recognized as one of the most aggressive types of human cancer, arising from the uncontrolled growth of melanocytes. Recent research has emphasized the crucial role of non-coding RNAs in the development and progression of cancer. In this study, we investigated the expression patterns of lncRNA MHENCR, miR-425 and PI3K in 17 melanoma samples. </span><b><span style="color:black">Methods:</span></b> <span style="color:black">&nbsp;In present study, we used real-time PCR to assess the expression levels of lncRNA MHENCR, miR-425 and PI3K in the serum of Iranian melanoma patients.</span> <span style="background:white"><span style="color:#172b4d">&beta;-actin and GAPDH were both used as controls.</span></span><br> <b><span style="color:black">Results:</span></b> <span style="color:black">Real-time PCR analysis showed that the expression of lncRNA MHENCR in the serum samples of melanoma patients was increased 1.23-fold compared to the control group </span><span style="background:white"><span style="color:#172b4d">(p < 0.05), </span></span><span style="color:black">&nbsp;while the expression of miR-425 and PI3K was decreased</span><span style="background:white"><span style="color:#172b4d"> by 0.828-fold and 0.795-fold, respectively (p < 0.05).</span></span><br> <b><span style="color:black">Conclusion:</span></b> <span style="color:black">Changes in the expression of </span><span style="color:black">MHENCR, miR-425 and PI3K </span><span style="color:black">highlight their key roles in melanoma-related regulatory pathways and their potential as diagnostic, prognostic, and therapeutic targets. </span></span></span></span></span><br> &nbsp; Melanoma, MicroRNA, lncRNA, miR-425, PI3K. 32 32 http://journal.zums.ac.ir/browse.php?a_code=A-10-7333-2&slc_lang=en&sid=1 Fatemeh Shahmohamadi zos.013640@gmail.com 5200319475328460089056 5200319475328460089056 No National center for health insurance research, Tehran, Iran. Zohre Sadeghi Z.saadeghi16@gmail.com 5200319475328460089057 5200319475328460089057 No Cellular and Molecular Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran. Zahra Davoudi davoudibiotech@gmail.com 5200319475328460089058 5200319475328460089058 Yes Department of Medical Biotechnology, Zanjan University of Medical Science, Zanjan, Iran