Background and Objectives: Nowadays, The knowledge management is an undeniable necessity so that without the knowledge management, organizations are nearly repulsive of competition and survival. The main purpose of the present study was to determine the relation between organizational culture and the knowledge management in teaching hospitals of Zanjan university of medical sciences. Materials and Methods: This was a descriptive study which carried out in 3 teaching hospitals of Zanjan university of medical sciences (Vali-Asr, Ayatollah Mousavi and Shahid Beheshti). Statistical population of this study included the whole staff of hospitals with the level of B.A and upper education. 190 individuals were selected and replied the questionnaire which consisted of 3 parts: Demographic information, knowledge management and organization culture. SPSS 13 was used for analyzing the data. Results: The staff of hospitals offered an average grade to organization culture. Five features or organization culture was examined which the highest grade or concession was given to: "The organization belonging ", "management style", "individual creativity", "management support" and aggression tolerance". The knowledge management was measured weak by the staff. The acquired concession by 3 features of knowledge management respectively was: "knowledge transfer”,"knowledge creation" and "knowledge protection and saving". There was a positive meaningful relationship between the organization culture and the knowledge management (P=0.0006, r=0.43). The most correlation relation was between the management supports, the individual creatively, the management style, the organization belonging and the aggression tolerance with knowledge management. Conclusion: Strong and dynamic organization culture is one of the main tools for successful manipulation of the knowledge management in organizations. Changes in organization culture in order to establish knowledge management must be paid more attention and support the knowledge, personal creativity development and management style.