یکشنبه 24 خرداد 1405
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Background & Objective: Enterobacteriaceae, a family of Gram-negative bacteria in the normal gut flora, are common human pathogens. The rising fecal carriage of carbapenemase-producing (CPE) and extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) poses significant resistance to beta-lactam antibiotics. Due to this issue, This study focused on the prevalence and molecular characterization of beta-lactamase genes (blaKPC, blaIMP, blaVIM, blaNDM, blaSHV, blaTEM, blaOXA, and blaCTX-M) in Enterobacteriaceae isolates from hospitalized patients in Shahriar, Iran.
Materials & Methods: A total of 85 stool samples were collected from hospitalized patients in the ICU and general wards of Imam Sajjad Hospital, Shahriar. Bacterial isolates were identified using standard microbiological methods for identification of Enterobacteriaceae. Antibiotic susceptibility profile of isolates was analysed by using the disc diffusion, and Minimum inhibitory concentrations (MICs) method based on the Clinical and Laboratory Standards Institute (CLSI) 2023 guidelines. Phenotypic detection of ESBL and such as metallo-beta-lactamase (MBL) resistance, was performed using combined disc tests with specific inhibitors. PCR amplification with specific primer was used for detection beta-lactamase genes including blaKPC, blaIMP, blaVIM, blaNDM, blaSHV, blaTEM, blaOXA and blaCTX-M.
Results: From 85 samples, 19 isolates of Klebsiella pneumoniae (20.43%) and 66 isolates of Escherichia coli (79.57%) were identified. Among these, 31 isolates exhibited ESBL and 10 were identified as MBL. Among the 31 carbapenem-resistant or intermediate isolates, all carried the blaCTX-M gene. Additionally, 2 isolates carried the blaNDM-1 gene, and another 2 harbored the blaIMP gene. However, the blaKPC and blaVIM genes were not detected in any of the isolates.
Conclusion: blaCTX-M gene is one of the most common resistant genes in Iran. According to studies, the prevalence of antibiotic resistance in Iran is rising dramatically, which reduces the choice of antibiotics to treat severe infections in the future.
Materials & Methods: A total of 85 stool samples were collected from hospitalized patients in the ICU and general wards of Imam Sajjad Hospital, Shahriar. Bacterial isolates were identified using standard microbiological methods for identification of Enterobacteriaceae. Antibiotic susceptibility profile of isolates was analysed by using the disc diffusion, and Minimum inhibitory concentrations (MICs) method based on the Clinical and Laboratory Standards Institute (CLSI) 2023 guidelines. Phenotypic detection of ESBL and such as metallo-beta-lactamase (MBL) resistance, was performed using combined disc tests with specific inhibitors. PCR amplification with specific primer was used for detection beta-lactamase genes including blaKPC, blaIMP, blaVIM, blaNDM, blaSHV, blaTEM, blaOXA and blaCTX-M.
Results: From 85 samples, 19 isolates of Klebsiella pneumoniae (20.43%) and 66 isolates of Escherichia coli (79.57%) were identified. Among these, 31 isolates exhibited ESBL and 10 were identified as MBL. Among the 31 carbapenem-resistant or intermediate isolates, all carried the blaCTX-M gene. Additionally, 2 isolates carried the blaNDM-1 gene, and another 2 harbored the blaIMP gene. However, the blaKPC and blaVIM genes were not detected in any of the isolates.
Conclusion: blaCTX-M gene is one of the most common resistant genes in Iran. According to studies, the prevalence of antibiotic resistance in Iran is rising dramatically, which reduces the choice of antibiotics to treat severe infections in the future.
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