پذیرفته شده و در حال انتشار                   برگشت به فهرست مقالات | برگشت به فهرست نسخه ها

XML English Abstract Print

Polymorphism in the Toll-like Receptor 2 Subfamily and Risk of Atopy: A population study in Zanjan, Iran
چکیده:   (58 مشاهده)

Background and Objective: Atopy is an allergic state with manifestations of asthma, atopic dermatitis and allergic rhinitis. There was genetic tendency to atopy and the genetic polymorphisms in the TLRs had been shown as a causal factor. The aim of this study was to investigate the association between TLR2 rs7656411 polymorphisms and atopic people in Zanjan Province.
Material and Methods: Total genomic DNA was extracted from the whole blood of atopic and non-atopic individuals (100 atopic patients and 50 healthy control subjects) that randomly selected in Zanjan. PCR amplification and direct sequencing methods were used for genotyping of TLR2 rs7656411 in all patients and controls.
Results: In this study, the GG genotype was higher in the patients than controls (15% vs 8% in non-atopic subjects). However, there was not significant (P-value= 0.21). We also didn’t find any significant difference between the frequency of alleles (G and T) in the patients and controls.
Conclusion: Our study showed that TLR2 rs7656411 polymorphisms are not significantly associated with atopy in an Iranian population, but the GG genotype might be a risk factor for atopy.

     
نوع مطالعه: مقاله پژوهشی | موضوع مقاله: Medical Biology
دریافت: 1404/1/23 | پذیرش: 1404/6/22 | انتشار: 1404/7/7
فهرست منابع
1. Grundy J, Matthews S, Bateman B, Dean T, Arshad SH. Rising prevalence of allergy to peanut in children: Data from 2 sequential cohorts. J Allergy Clin Immunol. 2002;110(5):784-9. [DOI:10.1067/mai.2002.128802] [PMID]
2. Ahmadiafshar A, Parchegani MR, Moosavinasab N, Koosha A. A Study of Relation between BCG Scar and Atopy in Schoolchildren of Zanjan City. Iran J Allergy Asthma Immunol. 2005;4(4):185-8.
3. Bousquet J. Allergy as a global problem:'think globally, act globally'. Allergy. 2002;57(8):661-2. [DOI:10.1034/j.1398-9995.2002.02002.x] [PMID]
4. Xiang F, Zeng Z, Wang L, Yang YP, Zhang QX. Polymorphisms and AR: A Systematic Review and Meta-Analyses. Front Genet. 2022;13:899923. [DOI:10.3389/fgene.2022.899923] [PMID] [PMCID]
5. Antonino M, Nicolo M, Jerome Renee L, Federico M, Chiara V, Stefano S, et al. Single-nucleotide polymorphism in chronic rhinosinusitis: A systematic review. Clin Otolaryngol. 2022;47(1):14-23. [DOI:10.1111/coa.13870] [PMID]
6. Dytiatkovskyi V, Drevytska T, Lapikova-Bryhinska T, Dosenko V, Abaturov O. Genotype Associations with the Different Phenotypes of Atopic Dermatitis in Children. Acta Medica (Hradec Kralove). 2021;64(2):96-100. [DOI:10.14712/18059694.2021.17] [PMID]
7. Bégin P, Nadeau KC. Epigenetic regulation of asthma and allergic disease. Allergy Asthma Clin Immunol. 2014;10(1):27. [DOI:10.1186/1710-1492-10-27] [PMID] [PMCID]
8. Sunyer J, Anto J, Castellsague J, Soriano J, Roca J. Total serum IgE is associated with asthma independently of specific IgE levels. The Spanish Group of the European Study of Asthma. Eur Respir J. 1996;9(9):1880-4. [DOI:10.1183/09031936.96.09091880] [PMID]
9. Riiser A. The human microbiome, asthma, and allergy. Allergy Asthma Clin Immunol. 2015;11:35. [DOI:10.1186/s13223-015-0102-0] [PMID] [PMCID]
10. van Ree R, Hummelshøj L, Plantinga M, Poulsen LK, Swindle E. Allergic sensitization: host-immune factors. Clin Transl Allergy. 2014;4(1):12. [DOI:10.1186/2045-7022-4-12] [PMID] [PMCID]
11. Hammad H, Chieppa M, Perros F, Willart MA, Germain RN, Lambrecht BN. House dust mite allergen induces asthma via Toll-like receptor 4 triggering of airway structural cells. Nat Med. 2009;15(4):410-6. [DOI:10.1038/nm.1946] [PMID] [PMCID]
12. Liu G, Zhao Y. Toll‐like receptors and immune regulation: their direct and indirect modulation on regulatory CD4+ CD25+ T cells. Immunology. 2007;122(2):149-56. [DOI:10.1111/j.1365-2567.2007.02651.x] [PMID] [PMCID]
13. Takeda K, Akira S. Roles of Toll‐like receptors in innate immune responses. Genes to Cells. 2001;6(9):733-42. [DOI:10.1046/j.1365-2443.2001.00458.x] [PMID]
14. Gangloff SC, Guenounou M. Toll-like receptors and immune response in allergic disease. Clin Rev Allergy Immunol. 2004;26(2):115-25. [DOI:10.1007/s12016-004-0006-0] [PMID]
15. Dai J, Liu B, Li Z. Regulatory T cells and Toll-like receptors: what is the missing link? Int Immunopharmacol. 2009;9(5):528-33. [DOI:10.1016/j.intimp.2009.01.027] [PMID] [PMCID]
16. Liu H, Komai-Koma M, Xu D, Liew FY. Toll-like receptor 2 signaling modulates the functions of CD4+ CD25+ regulatory T cells. Proc Natl Acad Sci U S A. 2006;103(18):7048-53. [DOI:10.1073/pnas.0601554103] [PMID] [PMCID]
17. Nyirenda MH, O'Brien K, Sanvito L, Constantinescu CS, Gran B. Modulation of regulatory T cells in health and disease: role of toll-like receptors. Inflamm Allergy Drug Targets. 2009;8(2):124-9. [DOI:10.2174/187152809788462581] [PMID]
18. Schaub B, Campo M, He H, Perkins D, Gillman MW, Gold DR, et al. Neonatal immune responses to TLR2 stimulation: influence of maternal atopy on Foxp3 and IL-10 expression. Respir Res. 2006;7(40):1-9. [DOI:10.1186/1465-9921-7-40] [PMID] [PMCID]
19. Bellinghausen I, König B, Böttcher I, Knop J, Saloga J. Regulatory activity of human CD4+ CD25+ T cells depends on allergen concentration, type of allergen and atopy status of the donor. Immunology. 2005;116(1):103-11. [DOI:10.1111/j.1365-2567.2005.02205.x] [PMID] [PMCID]
20. Ling EM, Smith T, Nguyen XD, Pridgeon C, Dallman M, Arbery J, et al. Relation of CD4+CD25+ regulatory T-cell suppression of allergen-driven T-cell activation to atopic status and expression of allergic disease. Lancet. 2004;363(9409):608-15. [DOI:10.1016/S0140-6736(04)15592-X] [PMID]
21. Schaub B, Liu J, Höppler S, Haug S, Sattler C, Lluis A, et al. Impairment of T-regulatory cells in cord blood of atopic mothers. J Allergy Clin Immunol. 2008;121(6):1491-9, 9.e1-13. [DOI:10.1016/j.jaci.2008.04.010] [PMID]
22. Kang I, Oh Y-K, Lee SH, Jung HM, Chae S-C, Lee JH. Identification of polymorphisms in the Toll-like receptor gene and the association with allergic rhinitis. Eur Arch Otorhinolaryngol. 2010;267(3):385-9. https://doi.org/10.1007/s00405-009-1100-y [DOI:10.1007/s00405-009-1103-8] [PMID]
23. Kormann M, Ferstl R, Depner M, Klopp N, Spiller S, Illig T, et al. Rare TLR2 mutations reduce TLR2 receptor function and can increase atopy risk. Allergy. 2009;64(4):636-42. [DOI:10.1111/j.1398-9995.2008.01891.x] [PMID]
24. Lazarus R, Raby BA, Lange C, Silverman EK, Kwiatkowski DJ, Vercelli D, et al. TOLL-like receptor 10 genetic variation is associated with asthma in two independent samples. Am J Respir Crit Care Med. 2004;170(6):594-600. [DOI:10.1164/rccm.200404-491OC] [PMID]
25. Arbour NC, Lorenz E, Schutte BC, Zabner J, Kline JN, Jones M, et al. TLR4 mutations are associated with endotoxin hyporesponsiveness in humans. Nat Genet. 2000;25(2):187-91. [DOI:10.1038/76048] [PMID]
26. Eder W, Klimecki W, Yu L, Von Mutius E, Riedler J, Braun-Fahrländer C, et al. Toll-like receptor 2 as a major gene for asthma in children of European farmers. J Allergy Clin Immunol. 2004;113(3):482-8. [DOI:10.1016/j.jaci.2003.12.374] [PMID]
27. Weidinger S, Novak N, Klopp N, Baurecht H, Wagenpfeil S, Rummler L, et al. Lack of association between Toll-like receptor 2 and Toll-like receptor 4 polymorphisms and atopic eczema. J Allergy Clin Immunol. 2006;118(1):277-29. [DOI:10.1016/j.jaci.2006.04.034] [PMID]
28. Kormann MS, Depner M, Hartl D, Klopp N, Illig T, Adamski J, et al. Toll-like receptor heterodimer variants protect from childhood asthma. J Allergy Clin Immunol. 2008;122(1):86-92. e1-e 8. [DOI:10.1016/j.jaci.2008.04.039] [PMID]
29. Novak N, Yu CF, Bussmann C, Maintz L, Peng WM, Hart J, et al. Putative association of a TLR9 promoter polymorphism with atopic eczema. Allergy. 2007;62(7):766-72. [DOI:10.1111/j.1398-9995.2007.01358.x] [PMID]
30. Solé X, Guinó E, Valls J, Iniesta R, Moreno V. SNPStats: a web tool for the analysis of association studies. Bioinformatics. 2006;22(15):1928-9. [DOI:10.1093/bioinformatics/btl268] [PMID]
31. Aryan Z, Rezaei N. Toll-like receptors as targets for allergen immunotherapy. Curr Opin Allergy Clin Immunol. 2015;15(6):568-74. [DOI:10.1097/ACI.0000000000000212] [PMID]
32. Reijmerink NE, Bottema RW, Kerkhof M, Gerritsen J, Stelma FF, Thijs C, et al. TLR‐related pathway analysis: novel gene-gene interactions in the development of asthma and atopy. Allergy. 2010;65(2):199-207. [DOI:10.1111/j.1398-9995.2009.02111.x] [PMID]
33. Santana NdL, Rêgo JL, Oliveira JM, Almeida LFd, Braz M, Machado LMM, et al. Polymorphisms in genes TLR1, 2 and 4 are associated with differential cytokine and chemokine serum production in patients with leprosy. Mem Inst Oswaldo Cruz. 2017;112(4):260-8. [DOI:10.1590/0074-02760160366] [PMID] [PMCID]
34. Wu Z, Xu J, Sun F, Chen H, Wu Q, Zheng W, et al. Single nucleotide polymorphisms in the toll-like receptor 2 (TLR2) gene are associated with microscopic polyangiitis in the northern Han Chinese population. Mod Rheumatol. 2015;25(2):224-9. [DOI:10.3109/14397595.2014.950034] [PMID]
35. Torres-García D, Cruz-Lagunas A, Figueroa MCG-S, Fernández-Plata R, Baez-Saldaña R, Mendoza-Milla C, et al. Variants in toll-like receptor 9 gene influence susceptibility to tuberculosis in a Mexican population. J Transl Med. 2013;11:220. [DOI:10.1186/1479-5876-11-220] [PMID] [PMCID]
36. Koponen P, Vuononvirta J, Nuolivirta K, Helminen M, He Q, Korppi M. The association of genetic variants in toll-like receptor 2 subfamily with allergy and asthma after hospitalization for bronchiolitis in infancy. Pediatr Infect Dis J. 2014;33(5):463-6. [DOI:10.1097/INF.0000000000000253] [PMID]
37. Liu J, Radler D, Illi S, Klucker E, Turan E, von Mutius E, et al. TLR2 polymorphisms influence neonatal regulatory T cells depending on maternal atopy. Allergy. 2011;66(8):1020-9. [DOI:10.1111/j.1398-9995.2011.02573.x] [PMID]
38. Zuo L, Lucas K, Fortuna CA, Chuang CC, Best TM. Molecular Regulation of Toll-like Receptors in Asthma and COPD. Front Physiol. 2015;6:312. [DOI:10.3389/fphys.2015.00312] [PMID] [PMCID]
39. Qian FH, Zhang Q, Zhou LF, Jin GF, Bai JL, Yin KS. Polymorphisms in the toll-like receptor 2 subfamily and risk of asthma: a case-control analysis in a Chinese population. J Investig Allergol Clin Immunol. 2010;20(4):340-6.
بازنشر اطلاعات
Creative Commons License این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.