Autism is a neurodevelopmental disorder that affects social interaction, communication, and behavior. This study aims to compare the autism patient group with a control group in terms of gender and age distribution, as well as assess the levels of calcium and Vitamin D3 in both groups. The study was conducted on 50 autism patients and 50 individuals in the control group. Data related to gender and age group were collected, along with measurements of calcium and Vitamin D3 levels in the blood. So, it was approved to conduct the study by Thi Qar Health Department the Research and Department of the College of Pharmacy / University of Thi-Qar. This study conducted from 11/2024 to 4/2025, at Thi-Qar Rehabilitation Center for autistic disorders. The results showed significant differences between the two groups. The proportion of males in the autism group was higher (74%) compared to the control group (42%). The most represented age group in the autism patients was 7-10 years (42%), while the most represented age group in the control group was 11 years and older (50%). Additionally, the study found a significant decrease in both calcium and Vitamin D3 levels in autism patients compared to the control group. The findings suggest a significant relationship between calcium and Vitamin D3 deficiencies in autism patients, which may contribute to the severity of the symptoms.