fa همسانه سازی جایگاه کاتالیتیک اگزوتوکسین A سودوموناس آیروژینوزا مقاله پژوهشی Original Research Article <p>Background and Objective: Antibody against Pseudomonas aeruginosa exotoxin A can be used in immunotherapy together with antibiotics to treat acute burn patients. Exotoxin A is one of the virulence factors in Pseudomonas aeruginosa that comprises of three domains, binding domain, translocation and catalytic domain. The purpose of this study was to produce recombinant domain of the catalytic part of this microorganism in order to produce antibody against it. Methods and Materials: Pseudomonas aeruginosa samples were isolated from burn patients hospitalized in Mousavi Hospital, Zanjan, Iran and Pseudomonas aeruginosa species were identified by Biochemical tests. Bacteria genomic DNA was extracted and exotoxin A gene determined by PCR. Catalytic domain of exotoxin A was amplified by PCR. Products and plasmid extracts was digested by restriction enzymes. Subsequently PCR products and plasmids transformed into E. coli BL21 (DE3). Clones containing gene of interest was determined by PCR, restriction enzyme and sequencing. Results: The sequence homology of the catalytic domain of exotoxin A was compared with that of the published gene data bank. The results showed a complete homology between our gene species and the published genome in data banks. Conclusion: The results of this study showed that about 90% of the isolated bacteria contained exotoxin A and there was a sequence homology between our species and published gene data banks.</p> Key word: Pseudomonas aeruginosa, Cloning, Exotoxin A, Domain catalytic 24 33 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-532&slc_lang=fa&sid=1 Bahram Amini بهرام امینی 5200319475328460060297 5200319475328460060297 No Science and Research Branch, Islamic Azad University, Tehran, Iran Mehdi Kamali مهدی کمالی mehkamali@bmsu.ac.ir 5200319475328460060298 5200319475328460060298 Yes Nanobiotechnology Research Center, Baqyiatallah University of Medical Sciences, Tehran, Iran Ali Zarei Mahmod abadi علی زارعی محمودآبادی 5200319475328460060299 5200319475328460060299 No Dept. of Biochemistry, Baqyiatallah University of Medical Sciences, Tehran, Iran Yousef Mortazavi یوسف مرتضوی 5200319475328460060300 5200319475328460060300 No Dept. of Molecular Medicine and Genetics, Zanjan University of Medical Sciences, Zanjan, Iran Azadeh Ebrahim habibi آزاده ابراهیم حبیبی 5200319475328460060301 5200319475328460060301 No Dept. of Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran Ebrahim Bayat ابراهیم بیات 5200319475328460060302 5200319475328460060302 No Science and Research Branch, Islamic Azad University, Tehran, Iran Nima Farhadi نیما فرهادی 5200319475328460060303 5200319475328460060303 No Nanobiotechnology Research Center, Baqyiatallah University of Medical Sciences, Tehran, Iran Hamidreza Javadi حمیدرضا جوادی 5200319475328460060304 5200319475328460060304 No Nanobiotechnology Research Center, Baqyiatallah University of Medical Sciences, Tehran, Iran Amir Homayon kyhan امیر همایون کیهان 5200319475328460060305 5200319475328460060305 No Nanobiotechnology Research Center, Baqyiatallah University of Medical Sciences, Tehran, Iran