fa بررسی تاثیر غلظت‌های مختلف CO2 در بیان ژن HSP70 در قارچ‌های درماتوفیت ترایکوفایتون روبروم و میکروسپوروم کانیس مقاله پژوهشی Original Research Article <p style="text-align: justify;"><strong><em>Background and Objectivs:</em></strong> <em>Trichophyton rubrum</em> is considered as the most common causes of dermatophytosis in human skin and nail tissues. <em>Microsporeum canis</em> is a zoophile dermatophyte which can be transmited to human. HSP70 is a 70&nbsp;KD heat shock protein in fungi. In this study, the effects of variable CO₂ concentrations were examined on HSP70 expression in <em>T. rubrum</em> and <em>M. canis</em>.<br> <strong><em>Materials and Methods:</em></strong> Strains used in this study were obtained from skin scales and nails of the patients who were suffering from onychomycosis. Samples were cultured on Sabouraud dextrose broth (SDB) and incubated at 25&deg;C for 2, 4 and 7 days under 3%, 5%, and 10% of CO₂ concentrations. Control cultures maintained for 7 days without CO₂concentrations. Then, RNA was isolated from the harvested mycelia mass, and HSP70 gene expression was studied in <em>T. rubrum</em> and <em>M. canis</em> by RT-PCR. The obtained results were compared to the Beta actin as a house keeping gene.<br> <strong><em>Results:</em></strong> The results of this study revealed the maximum variations under 3%,5%, and 10% of CO₂ concentrations in maximum 7 days incubation period, and the expression of HSP70 gene showed different&nbsp; variations&nbsp; under different CO₂ concentrations.<br> <strong><em>Conclusion:</em></strong> Our results showed a negative effect of CO₂concentrations in the expression of HSP70 in <em>T. Rubrum </em>and a positive effect in <em>M. canis </em>comparing to the controls<strong>. </strong></p> Dermatophyte, Trichophyton rubrum, Microsporeum canis, HSP70 Gene 25 36 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-564&slc_lang=fa&sid=1 Sima Darabian سیما دارابیان dsima2004@yahoo.com 5200319475328460060174 5200319475328460060174 Yes School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran کارشناس ارشد قارچ شناسی پزشکی، عضو هیئت علمی دانشگاه علوم پزشکی زنجان Sasan Rezaie ساسان رضایی 5200319475328460060175 5200319475328460060175 No Division of Molecular Biology, Dept. of Medical Mycology & Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran دکترای تخصصی قارچ شناسی پزشکی، دانشیار دانشگاه علوم پزشکی تهران Parinoush Kordbachche پریوش کردبچه 5200319475328460060176 5200319475328460060176 No Division of Molecular Biology, Dept. of Medical Mycology & Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran دکترای تخصصی قارچ شناسی پزشکی، استاد دانشگاه علوم پزشکی تهران Mohammad Reza Safari محمد رضا صفری 5200319475328460060177 5200319475328460060177 No Division of Molecular Biology, Dept. of Medical Mycology & Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran کارشناش علوم آزمایشگاهی، دانشگاه علوم پزشکی تهران