fa مقایسه و ارزیابی تولید پروتئین‎های دفعی- ترشحی پروتواسکولکس اکینوکوکوس گرانولوزوس در محیط‎های کشتRPMI ، DMEM و PBS غنی شده با گلوکز مقاله پژوهشی Original Research Article <p style="text-align: justify;"><strong><em>Background and Objective:</em></strong> Preparation of proper antigens is an important issue in serology of hydatidosis. Investigators have been able to obtain excretory/secretory antigens (E/S Ags) by short-term culture of <a name="OLE_LINK4"></a><a name="OLE_LINK3">protoscoleces</a> in a couple of culture media. However, no data are available about production rate of such antigens in different culture media. The present study was carried out to evaluate the production of E/S Ags (proteins) in PBS complemented with glucose, DMEM and RPMI culture media.<br> <strong><em>Material and Methods</em></strong>: To obtain E/S proteins, protoscoleces of echinococcus were cultured in PBS complemented with 10% glucose, RPMI and DMEM for 72 hours. Proteins secreted in culture media were concentrated and assayed. To characterize different components, proteins were electrophoresed on SDS-PAGE. Data were analyzed using One-way ANOVA and Tukey HSD tests.<br> <strong><em>Results</em></strong><strong>:</strong> The mean concentration of E/S proteins in PBS medium in 24 hours of culture was significantly higher than DMEM and RPMI (<em>P</em><0.05). However, such a difference was not observed between E/S proteins in DMEM and RPMI media. E/S proteins obtained from PBS medium were separated into 12 major bands and the two other media into 14 major bands within a range of molecular masses of 16 to 67 kDa.<br> <strong><em>Conclusion</em></strong><strong>:</strong> PBS complemented with glucose is more appropriate than the two other media for E/S proteins production. The best time to obtain E/S proteins is the first 48 hours of culture.</p> Echinococcus granulosus, Protoscoleces, Excretory/Secretory antigens 44 53 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-567&slc_lang=fa&sid=1 Ali Haniloo علی هانیلو 5200319475328460060178 5200319475328460060178 No Dept. of Parasitology and Entomology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. دکترای تخصصی انگل‌شناسی، دانشیار دانشگاه علوم پزشکی زنجان Faezeh Najafi فائزه نجفی faezeh_najafi@yahoo.com 5200319475328460060179 5200319475328460060179 Yes Dept. of Parasitology and Entomology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. کارشناس ارشد انگل‌شناسی، دانشکده پزشکی دانشگاه علوم پزشکی زنجان Asghar Fazaeli اصغر فضائلی 5200319475328460060180 5200319475328460060180 No Dept. of Parasitology and Entomology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. دکترای تخصصی انگل‌شناسی، دانشیار دانشگاه علوم پزشکی زنجان Ali Abbas Nourian عباس علی نوریان 5200319475328460060181 5200319475328460060181 No Dept. of Parasitology and Entomology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. دکترای تخصصی انگل‌شناسی، دانشیار دانشگاه علوم پزشکی زنجان