fa اثرات سفیکسیم بر بافت شناسی بیضه و هورمون‌های محور هیپوفیز- گناد در موش بالغ نژادBalb/C مقاله پژوهشی Original Research Article <p style="text-align: justify;"><strong><em>Background and Objective:</em></strong> Cefixime is an antimicrobial agent which has a widespread ability against various pathogens, especially gram-negative organisms. Today, physicians apply cephalosporins especially cefixime in a wide scale. Regarding the side effects of some of these antibiotics on reproductive system, this study was conducted to determine the effect of cefixime on pituitary- gonadal hormones, gonadotrophins and testes morphology in adult male mice.<br> <strong><em>Materials and Methods:</em></strong> Eighteen male mice (age: 12-16 weeks, weight: 35<img alt="" height="16" src="file:///C:/Users/vahab/AppData/Local/Temp/msohtmlclip1/01/clip_image002.gif" width="15" >5 gr) were divided into three groups; control, sham and experimental (6 mice in each group). Experimental group received cefixime<br> (0.5 gr/kg<span dir="RTL">/</span>day) as a solution in dimethyl solfoxide (DMSO) for 10 days; the sham group received only drug solvent (DMSO) via IP injection and the control group remained intact<span dir="RTL">.</span> The animals were weighed and sacrificed. Level of hormones was measured by Radioi Immuno Assay (RIA) method. Then, tissues were fixed in Buin&#39;s fixative. Sections were cut into 5 &micro;m thicknesses and stained with Hematoxylin and Eosin (H& E). Data were analyzed using T-test and SPSS software.<span dir="RTL"></span><br> <strong><em>Results:</em></strong> Count of spermatogenic, Sertoli and leydig cells and titer of FSH significantly decreased in the experimental group in comparison with the control and sham groups (P<0.01 and P<0.05). In the experimental group, DHEA hormone decreased significantly (P<0.05) in comparison with sham. No significant differences were seen in other factors between the groups.<br> <strong><em>Conclusion:</em></strong> Regarding physiological role of Sertoli cells during spermatogenesis, reduction of FSH hormone may lead to negative effects on the <a name="OLE_LINK18"></a><a name="OLE_LINK17">sperm production and reproductive potential of male mice</a>.</p> Cefixime, Testosterone, Gonadotrophins, Testis, Mice 54 62 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-568&slc_lang=fa&sid=1 Zohreh AHmadi زهره احمدی ramezani@mail.aiau.ac.ir 5200319475328460049736 5200319475328460049736 Yes Dept of Biology, Payamenoor University-Center of Tehran, Tehran, Iran کارشناسی ارشد زیست شناسی، دانشگاه پیام نور مرکز تهران Mina Ramezani مینا رمضانی 5200319475328460049737 5200319475328460049737 No Dept of Biology, Islamic Azad University, Ashtian Branch, Ashtian, Iran دکترای زیست شناسی جانوری، استادیار دانشگاه آزاد اسلامی واحد آشتیان Davood Sohrabi داود سهرابی 5200319475328460049738 5200319475328460049738 No Dept of Anatomy, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دکترای علوم تشریح، استادیار دانشگاه علوم پزشکی زنجان