fa اثرات دوگانه مرفین بر روی فعالیت‌های صرعی خود بخودی در برش‌های هیپوکامپ مغز موش مقاله پژوهشی Original Research Article <p style="text-align: justify;"><strong><em>Background and Objective:</em></strong> Opiates have complex effects on seizure activity. They have both anti- and proconvulsive effects depending on experimental conditions.<br> The aim of this study was to determine the effects of different doses of morphine and naloxon on spontaneous seizure activity in mouse brain hippocampal slices.<br> <strong><em>Materials and Methods:</em></strong> Spontaneous epileptic activity in the brain hippocampal slices of mouse was induced by continuous perfusion of low magnesium artificial cerebrospinal fluid (low -Mg²⁺ACSF). Extra cellular recordings were performed in the hippocampal CA1 pyramidal cell layer to account for the effects of the drugs on amplitude, duration and number of the ictal events as well as number of interictal spikes.&nbsp;<br> <strong><em>Results:</em></strong> Application of morphine had a biphasic effect on the recorded spontaneous seizure-like events. In a low concentration (10 &micro;M), morphine decreased seizure activity. Higher morphine concentrations (30 & 100 &micro;M) enhanced seizure activity in an apparent dose-dependent manner. Naloxone, a nonselective opiate antagonist, blocked the proconvulsant action of morphine.<br> <strong><em>Conclusion:</em></strong> The results of this study showed that the effect of morphine on seizure in mouse is dose dependent. In other words, low systemic doses of morphine have anticonvulsant effects while high doses are proconvulsant.</p> Morphine, Seizure, Hippocampal CA1, Low- Mg2+ ACSF 37 47 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-578&slc_lang=fa&sid=1 Mohhamad Hossein Esmaeili محمدحسین اسماعیلی Esmail66@yahoo.com 5200319475328460060150 5200319475328460060150 Yes Dept. of Physiology, Qazvine University of Medical Sciences, Qazvine, Iran دکترای تخصصی فیزیولوژی، استادیار دانشگاه علوم پزشکی قزوین Mahin Ganjkhani مهین گنج‌خانی 5200319475328460060151 5200319475328460060151 No Dept. of Physiology, Zanjan University of Medical Sciences, Zanjan, Iran دکترای تخصصی فیزیولوژی، استادیار دانشگاه علوم پزشکی زنجان Aliosat Mellati علی‌اوسط ملتی 5200319475328460060152 5200319475328460060152 No Dept. of Biochemistry, Zanjan University of Medical Sciences, Zanjan, Iran دکترای تخصصی بیوشیمی، دانشیار دانشگاه علوم پزشکی زنجان Hashem Haghdoost-Yazdi هاشم حق‌دوست یزدی 5200319475328460060153 5200319475328460060153 No Dept. of Physiology, Qazvine University of Medical Sciences, Qazvine, Iran دکترای تخصصی فیزیولوژی، استادیار دانشگاه علوم پزشکی قزوین Mohammad Sofiabadi محمد‌ صوفی آبادی 5200319475328460060154 5200319475328460060154 No Dept. of Physiology, Qazvine University of Medical Sciences, Qazvine, Iran دکترای تخصصی فیزیولوژی، استادیار دانشگاه علوم پزشکی قزوین