fa بررسی خصوصیات مولکولی و الگوی حساسیت آنتی بیوتیکی استافیلوکوکوس ارئوس مقاوم به متی سیلین در بیمارستان آموزشی شهید بهشتی کاشان مقاله پژوهشی Original Research Article <p style="text-align: justify;"><strong><em>B</em></strong><strong><em>ackground and Objective:</em></strong> Methicillin resistant <em>Staphylococcus aureus</em> (MRSA), which is the most common cause of nosocomial infection, has been a major cause of morbidity and mortality around the world. This study was carried out to find out the resistance pattern and staphylococcal cassette chromosome mec (SCC<em>mec</em>) typing among MRSA.<br> <strong><em>Materials and Methods: </em></strong>This descriptive work was done on 87 Methicillin resistant <em>Staphylococcus aureus</em> isolates which were collected from the patients with infections in a teaching hospital in Kashan, Iran, during the period of 2009. The isolates were tested for antibiotic resistance by the disc-diffusion method, covering 10 antimicrobials. The genotypes of SCC<em>mec</em> subtypes were determined by multiplex PCR.<br> <strong><em>Results:</em></strong> Amomg 87 MRSA isolated tested; the highest resistance was shown against erythromycin, clindamycin, sulfamethoxazole-trimethoprim, and tetracycline respectively. By contrast, the highest sensitivity was shown to amikacin. All of the isolates were resistant to Beta-lactams. All of the isolates were resistant to at least three classes of antibiotics, and all of the isolates were sensitive to vancomycin.<br> Three (3.4%) MRSA strains were SCC<em>mec</em> type I, 12 (13.8%) were type II, 8 (9.2%) were type IV-b, 4 (4.6%) were type IV-d, and 3 (3.4%) were typeV. Overall, 57 (65.5%) MRSA strains could not be typed.<strong><em> Conclusion:</em></strong> The rising trend of multi-resistance to antibiotics poses an alarming threat to treatment of MRSA infections. Our findings show that clinical isolates of MRSA in our hospital carrying various kinds of staphylococcal cassette chromosome mec (SCC<em>mec</em>) types. SCC<em>mec typse </em>II and IV were the predominant strain of MRSA identified.</p> Methicillin-resistant S. aureus, Antibiotic resistance, Multidrug resistance, Typing 31 40 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-603&slc_lang=fa&sid=1 Elham Zeinali الهام زینلی 5200319475328460060049 5200319475328460060049 No Dept. of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran REzvan Moniri رضوان منیری moniri@kaums.ac.ir 5200319475328460060050 5200319475328460060050 Yes Dept. of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Anatomical Sciences Research Center, Kashan, Iran. Seyed zgholam Abbas Mousavi سید غلام عباس موسوی 5200319475328460060051 5200319475328460060051 No Dept. of Biostatic, Faculty of Health, Kashan University of Medical Sciences, Kashan, Iran