fa بررسی اثر آنتی باکتریال غشای آمنیوتیک انسان در شرایط متفاوت زمانی، دمایی و pH کارآزمایی بالینی Clinical Trials <p>Background and Objective: Human amniotic membrane, which is the innermost layer of placenta, contains beta defensins and elafin, which both have antibacterial properties. These antibacterial activities depend on the conditions and environmental factors. The purpose of this study was to evaluate the effect of environmental factors on human amniotic membrane antibacterial properties against Klebsiella pneumonia (ATCC7881), Entrococcus faecalis (ATCC29212), Pseudomonas aeruginosa (ATCC27853), Salmonella enteric (BAA-708), and E. coli (ATCC25922) strains in vitro. Materials and Methods: The amniotic membrane samples were obtained from caesarean women in Imam Khomeini hospital. Participating women were all seronegative for HIV, HBV, HCV, and syphilis. The samples were cut into 1.5&times;1.5 cm pieces. The 0.5 McFarland bacterial suspensions were prepared and spread on Muller-Hinton agar medium, and a piece of membrane was placed in the centre of each plate. Samples were examined at different time intervals (24, 48, and 72 hr), temperatures (25, 33, 37&deg;C) and pH (6.5, 7, and 7.5) as variables. Results: The results show that time and pH as variable parameters did not affect the antibacterial properties of the amniotic membrane. However, the change in temperature (25 and 33&deg;C vs. 37&deg;C) had a significant impact on P. aeruginosa. Conclusion: Antibacterial properties of the amniotic membrane seem resistant against environmental factors, except for especial cases, and this sustainability could expand its usage in clinical procedures and different conditions.</p> Keywords: Human amniotic membrane, Antibacterial properties, Environmental factors 37 44 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-652&slc_lang=fa&sid=1 Mohmmad mehdi ُSoltan Dallal محمد مهدی سلطان دلال soltanirad34@yahoo.com 5200319475328460059799 5200319475328460059799 Yes Division of Microbiology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran,Antimicrobial Resistant Research Center, Tehran University of Medical Sciences, Tehran, Iran. Zohreh Kalafi زهره کلافی 5200319475328460059800 5200319475328460059800 No Dept. of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Zanjan, Iran Abdolaziz Rastegare Lari عبدالعزیز رستگار لاری 5200319475328460059801 5200319475328460059801 No Division of Microbiology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran,Antimicrobial Resistant Research Center, Tehran University of Medical Sciences, Tehran, Iran. Seyed Kazem Hosseini سید کاظم حسینی 5200319475328460059802 5200319475328460059802 No Transplant Bank, Imam Khomeini Hospital, Tehran, Iran Abbas Rahimi Foroushani عباس رحیمی فروشانی 5200319475328460059803 5200319475328460059803 No Dept. of Epidemiology and Biostatics, School of Public Health, Tehran University of Medical Sciences Tehran, Iran. Zahra Deilami Khiabani زهرا دیلمی خیابانی 5200319475328460059804 5200319475328460059804 No Biological Research Center, Islamic Azad University, Zanjan Branch, Zanjan, Iran. Farhad Nikkhahi فرهاد نیکخواهی 5200319475328460059805 5200319475328460059805 No Biological Research Center, Islamic Azad University, Zanjan Branch, Zanjan, Iran. Siamak Heidarzadeh سیامک حیدرزاده 5200319475328460059806 5200319475328460059806 No Division of Microbiology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran