fa تشخیص ژن‌های blaTEM-1، blaSHV-1، blaSHV-5، blaCTX-M-1، blaCTX-M-2، blaCTX-M-3، blaCTX-M-9، blaOXA-1، blaGES-1 و blaGES-2 درایزوله‌های پسودوموناس ائروژینوزا جدا شده از نمونه‌های بالینی و محیطی بیمارستان شهید بهشتی کاشان کارآزمایی بالینی Clinical Trials <p>Background and Objective: Pseudomonas aeruginosa is the most common pathogen causing nosocomial infections. The aim of this study was to determine the antimicrobial susceptibility pattern and prevalence of ESBLs in clinical and environmental isolates of P. aeruginosa by phenotypic and genotypic techniques. Materials and Methods: In this descriptive study, a total of 100 P. aeruginosa strains isolated from different clinical and environmental specimens were used. The antibiotic resistance pattern to eight antimicrobial agents was determined by disk diffusion method. The ESBLs producing strains were confirmed by double-disk-diffusion test, and the blaTEM-1, blaSHV-1, blaSHV-5, blaCTX-M-1, blaCTX-M-2, blaCTX-M-3, blaCTX-M-9, blaOXA-1, blaGES-1, and blaGES-2 genes were detected by PCR. Results: Piperacillin and ciprofloxacin showed the highest (36%) and the lowest (16%) resistance against the isolates, respectively. Thirty percent of the total isolates were resistant to at least three classes of antibiotics. By double-disk-diffusion test, eight strains (8%) were ESBL positive. According to the PCR results, the blaGES-2, blaSHV-1, blaSHV-5, and blaCTX-M-1 genes were detected in 8, 2, 2, and 1 isolates of ESBLs producing strains respectively. Conclusion: The blaGES-2 gene displayed an expanded hydrolysis profile to the antibiotic imipenem. In fact, this enzyme, which plays an important role in resistance to imipenem, was detected in all ESBLs producing P. aeruginosa strains. This is the first report describing blaGES-2 producing P. aeruginosa in Iran.</p> Keywords: ESBL, gene, Molecular detection, Multidrug resistant, Pseudomonas aeruginosa 42 51 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-664&slc_lang=fa&sid=1 Zahra Tavajjohi زهرا توجهی 5200319475328460059754 5200319475328460059754 No Dept. of Biology, Faculty of Sciences, Alzahra University, Tehran, Iran Rezvan Moniri رضوان منیری moniri@kaums.ac.ir 5200319475328460059755 5200319475328460059755 Yes Dept. of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Anatomical Sciences Research Center, Kashan, Iran Ahmad Khorshidi احمد خورشیدی 5200319475328460059756 5200319475328460059756 No Dept. of Microbiology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran Mehdi Rohani مهدی روحانی 5200319475328460059757 5200319475328460059757 No Pasteur Institute of Iran, Tehran, Iran