fa تعیین الگوی مقاومت اسینتوباکتر بومانی ایزوله شده از زخم بیماران سوختگی بستری در بیمارستان مطهری تهران کارآزمایی بالینی Clinical Trials <p>Background and Objective: Acinetobacter baumannii is an important human pathogen that has been a focus of attention in recent years. These bacteria are a leading cause of therapeutic resistant nosocomial infections especially in burned or hospitalized patients in intensive care Units. The aim of this study was to isolate the Acinetobacter baumannii species from the wounds of burned patients and to determine antimicrobial resistance pattern of these bacteria for selection of appropriate antibiotics. Materials and Methods: Samples were collected from patients and transferred to the laboratory under standard conditions. Bacteria were isolated and purified by conventional culture methods. Identification of bacterial species was performed by standard biochemical tests. The isolates that were identified as Acinetobacter baumannii were subsequently tested for antibiotic resistance by the disk diffusion agar method for 17 different antibiotics. The tests were carried out on Muller Hinton agar (MHA) plates and incubated at 35˚C for 18 hrs. The minimum inhibition concentrations were determined for 5 common therapeutic antibiotics. Results: Out of the 65 clinical Acinetobacter baumannii isolates collected, 61 (94%) were multi drug resistant (MDR). Ceftazidime and aztronam (98%) were the most effective antibiotics against Acinetobacter baumannii. To determine the MIC, the highest levels of antibiotic resistance were seen against ceftazidime, cefepime, and ciprofloxacin. Conclusion: Our results confirm the high prevalence of Acinetobacter baumannii resistant isolates and the ensuing therapeutic problems in Iran. Determination of the resistance patterns of these bacteria according to MIC is necessary, and it can be especially helpful in treatment of burned patients.</p> Keywords: Acinetobacter baumannii, Antibiotic resistant, Minimum inhibition concentration 112 119 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-682&slc_lang=fa&sid=1 Abdollah Ardebili عبدالله اردبیلی 5200319475328460059734 5200319475328460059734 No Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran. Iran Leila Azimi لیلا عظیمی 5200319475328460059735 5200319475328460059735 No Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran. Iran Hajar Mohammadi-Barzelighi هاجر محمدی برزلیقی 5200319475328460059736 5200319475328460059736 No Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran. Iran Parvaiz Owlia پرویز اولیا 5200319475328460059737 5200319475328460059737 No Dept. of Microbiology, School of Medicin, Shahed University, Tehran, Tran Maryam Beheshti مریم بهشتی 5200319475328460059738 5200319475328460059738 No Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran. Iran Maliheh Talebi ملیحه طالبی 5200319475328460059739 5200319475328460059739 No Dept. of Microbiology, School of Medicin, Tehran University of Medical Sciences, Tehran. Iran Mosadegh Jabbari مصدق جباری 5200319475328460059740 5200319475328460059740 No Dep.of Nephrology, Rasoul Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran Abdolaziz Rastegar Lari عبدالعزیز رستگار لاری lari@tums.ac.ir 5200319475328460059741 5200319475328460059741 Yes 1,2 Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran. Iran ,Dept. of Microbiology, School of Medicin, Tehran University of Medical Sciences, Tehran. Iran.