fa بررسی ارتباط بین کمبود پروتامین و آزاد شدن قطعات DNA از اسپرم‌های نگهداری شده در محیط مصرفی Ham’s F10 کارآزمایی بالینی Clinical Trials <p>Background and Objective: Protamine deficiency and DNA fragmentation have a profound effect on embryo gene expression. Incubation of sperm in culture medium leads to morphological changes of the nucleus and DNA release. However, the relationship between protamine deficiency and DNA fragments released from incubated sperm and the presence of the DNMT1 gene is not known. Materials and Methods: Semen analysis was performed according to the WHO criteria. CMA3 staining was used to determine protamine deficiency. Twenty million sperms per sample were incubated in Ham&rsquo;s F10 medium for 24 hr at 37 &deg;C. After centrifugation, DNA was extracted from the supernatant. DNA concentration was measured by biophotometer. The presence of the DNMT1 gene was confirmed by PCR using specific primers and agarose gel electrophoresis. Results:A significant correlation was observed between the DNA fragments released from sperm in culture media and CMA3 staining (P<0.05). The presence of DNMT1 gene was significantly correlated with DNA fragments released from the sperm (P<0.05). Protamine deficiency and the presence of DNMT1 gene were not significantly correlated. Conclusion:These results demonstrate that incubation of protamines-deficient sperm leads to the release of additional DNA fragments that eventually could have detrimental effectson embryo&rsquo;s health.</p> Keywords: Protamine, Sperm chromatin, DNA fragments 30 39 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-698&slc_lang=fa&sid=1 Mohammad Salehi محمد صالحی m.salehi@sbmu.ac.ir 5200319475328460059591 5200319475328460059591 Yes 1,2 Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Dept. of Biology and Anatomical sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.. Mozhdeh Gholizadeh مژده قلی‌زاده 5200319475328460059592 5200319475328460059592 No Dept. of Biology, Faculty of Basic Sciences, Islamic Azad University Science and Research Branch, Tehran, Iran Sedigheh Nematollahi صدیقه نعمت الهی 5200319475328460059593 5200319475328460059593 No Dept. of Transgenic Technology, Stem Cell Technology Research Center, Tehran, Iran Mozhgan Bandehpour مژگان بنده پور 5200319475328460059594 5200319475328460059594 No 1,5 Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran ,Dept. of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.. Bahram Kazemi Damaneh بهرام کاظمی دمنه 5200319475328460059595 5200319475328460059595 No 1,5 Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran ,Dept. of Biotechnology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. . Ahmad Hosseini احمد حسینی 5200319475328460059596 5200319475328460059596 No Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran