fa کاربرد بیوانفورماتیک و مهندسی ژن جهت طراحی کلون بهینه، برای افزایش تولید پروتیین فعال کننده‌ی نوتروفیل هلیکوباکترپیلوری در اشریشیا کلی کارآزمایی بالینی Clinical Trials <p>Background and Objective: As the main virulence factor of Helicobacter pylori, HP-NAP has an important role in immunoprotection against this pathogen. This antigen is a strong candidate as a part of multi-component vaccine in the clinical trial against this bacterium. Due to NAP importance, it was used in this study as a template for optimization of heterologous genes with a low A-T content and low expression in E. coli. Materials and Methods: A synthetic single gene that could reach the highest level of expression in the host was designed by using bioinformatics tools. Results: A number of factors that influence gene expression level were changed for HP-NAP gene optimizing: the codon usage bias in E. coli was changed the G+C content was upgraded from 38% to 45% and the stem-loop structure was broken. These could result to prolong of the half-life of the mRNA and overexpression of recombinant of HP-NAP protein up to 800 mg per liter. Conclusion: Applying of bioinformatics tools was appropriated to optimize of HP-NAP overexpression in E. coli. From our results, it appears that combination of In Silico and experimental approach is a logical approach for expression of heterologous genes in another host.</p> Keywords: Helicobacter pylori, Bioinformatics, Optimization, Recombinant HP-NAP, Cloning, Expression. 40 54 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-699&slc_lang=fa&sid=1 Mohammad Ali Haghighi محمدعلی حقیقی 5200319475328460059597 5200319475328460059597 No Dept. of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Ashraf Mohabati Mobarez اشرف محبتی مبارز mmmobarez@modares.ac.ir 5200319475328460059598 5200319475328460059598 Yes Dept. of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Hatef Salmanian هاتف علی سلیمانیان 5200319475328460059599 5200319475328460059599 No Dept. of Plant Biotechnology. National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran Mohammad Reza Zali محمد رضا زالی 5200319475328460059600 5200319475328460059600 No Research Center for Gastroenterology and Liver Diseases (RCGLD), University of Shahid Beheshti Medical Sciences, Tehran, Iran Seyed Mohammad Moazzeni سید محمد موذنی 5200319475328460059601 5200319475328460059601 No Dept. of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Ali Asghar Karkhane علی اصغر کارخانه 5200319475328460059602 5200319475328460059602 No Dept. of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran