Journal of Advances in Medical and Biomedical Research

fa شناسایی مولکولی فسفولیپاز D به عنوان عامل مؤثر در رشد و بیماری‌زایی میکروارگانیسم‌ها Identification of Phospholipase D Gene as a Contributing Factor in Growth and Virulence of Microorganisms Background and Objective: Secretory extracellular Phospholipases are generally involved in hydrolysis of extracellular phospholipids and thus providing nutritive source of carbon, nitrogen, and phosphate. However, intracellular phospholipases perform metabolic functions and adjust biologic activities. Synthesis of phospholipases in different pathogenic microorganisms and their mode of action in virulence of the microorganism have been the center of attention in recent studies. Materials and Methods: During this study using degenerate primers based on homologous amino acid sequences of phospholipase D (PLD) search for detection of Aspergillus fumigatus was carried out. DNA extraction of A. fumigatus was performed and then using degenerate primers based on nucleotide sequences of Phospholipase D gene was degenerated. Predicted 850 bp product from A. fumigatus was cloned in pGEMT-Easy vector and then transformed into E. coli Top 10 F’ competent cell for extraction of cloned DNA fragment. Results: Sequence analysis of 850 bp fragments revealed a sequence for the PLD gene of A. fumigatus with a high homology to published PLD sequences in other microorganisms. Conclusion: Gene sequence studies are generally conducted to determine the participation of gene expression in pathogenecity of microorganisms, the evaluation of biochemical features and physiologic function of gene product for understanding the basic knowledge to provide immunity, production of  vaccine, drug, or blocker for gene product, utilizing the gene product, utilizing the gene product for infection detection and so on. Thus, cloning of a part of phospholipase D gene in order to full identification of nucleotides sequence of this gene could contribute to achieve those goals. Virulence, Microorganisms, Phospholipase D, Aspergillus fumigatus 35 46 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-75&slc_lang=fa&sid=1 Abdolhasan Kazemi عبدالحسن کاظمی Kazemi1338@Gmail.com 5200319475328460057923 5200319475328460057923 Yes Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. دکترای تخصصی بیولوژی ملکولی، دانشیار دانشگاه تبریز Jeffery Robson جفری رابسون 5200319475328460057924 5200319475328460057924 No دکترای بیولوژی ملکولی، دانشیار دانشگاه منچستر Davide Denning دیوید دنینگ 5200319475328460057925 5200319475328460057925 No دکترای تخصصی بیماری‌های عفونی و فوق‌تخصصی میکوزهای ریوی، استاد دانشگاه منچستر