Journal of Advances in Medical and Biomedical Research

fa جداسازی سلول‌های بنیادی مزانشیمی از بافت چربی و ریه‌ی موش BALB/c و مقایسه‌ی ایمونوفنوتایپ آن‌ها Mesenchymal Stem Cell Isolation from Adipose Tissue and Lung of BALB/c Murine and Comparison of Their Immunophenotype کارآزمایی بالینی Clinical Trials <p>Background and Objective: Mesenchymal stem cells are promising sources of stem cells for tissue repair because of their ability to differentiate into different cells, easy proliferation and culture, and immunomodulatory properties. Despite extensive research on the immunophenotype of mesenchymal stem cells, a lack of specific markers comprises challenges for researchers. The aim of this research was to isolate and expand murine lung and adipose derived mesenchymal stem cells and to compare their expression of surface protein markers. Materials and Methods: Mesenchymal stem cells were isolated from murine lung and adipose tissues. Specific differentiation media were added for differential investigations. Finally, the expression of surface antigens were measured by flow cytometry and compared in both cell populations. Results: Mesenchymal stem cells from both tissues were proliferated well and adipocyte and osteocyte differentiations were observed. Moreover, they showed CD73, CD105 and CD90 expression, but no expression of CD34, CD45 and CD11b was observed. In this study, lung CD105+ mesenchymal stem cells possessed lower percentage than those in adipose tissue. Conclusion: Mesenchymal stem cells were isolated from lung similar to adipose derived mesenchymal stem cells and expressed common markers associated with adipose tissue mesenchymal stem cells.</p> Keywords: Mesenchymal stem cells, Adipose, Lung, BALB/c Murine, Immunophenotype 17 29 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-746&slc_lang=fa&sid=1 Zahra Hosseinpur زهرا حسین‌پور 5200319475328460059417 5200319475328460059417 No Dept. of Immunology, Faculty of Medical Sciences, Shahed University, Tehran, Iran Seyed Mahmoud Hashemi سید محمود هاشمی 5200319475328460059418 5200319475328460059418 No Dept. of Stem Cell Biology Pouyandegan Tissue Engineering Research Center, Tehran, Iran Issa Salehi عیسی صالحی 5200319475328460059419 5200319475328460059419 No Dept. of Immunology, Tehran University of Medical Sciences, Tehran, Iran Touba Ghazanfari طوبی غضنفری tghazanfari@yahoo.com 5200319475328460059420 5200319475328460059420 Yes Immunoregulation Research Center, Shahed University, Tehran, Iran