fa بررسی قدرت تشخیصی آزمون روزبنگال در مقایسه با الایزا در بروسلوز حاد و مزمن مقاله پژوهشی Original Research Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><strong><span style="font-size:18px;"><span style="color:#ffffff;"><span style="background-color:#16a085;">Background:</span></span></span></strong><span style="font-size:16px;"> Since Brucellosis has a high incidence in Iran, a quick, sensitive and specific diagnosis for immediate therapy is necessary. This study was conducted on patients with Brucellosis in the city of Zanjan in the years 2002-2003 to compare the diagnostic values of the rapid Rose Bengal test and the selective ELISA assay. </span><br> <strong><span style="font-size:18px;"><span style="color:#ffffff;"><span style="background-color:#16a085;">Materials and Methods:</span></span></span></strong><span style="font-size:16px;"> All patients that were suspicious of Brucellosis and were referred to the laboratory by specialists were entered to the study. In order to conduct Rose Bengal test and to measure the specific IgG and IgM antibodies by ELISA, 5 ml of blood was taken from each patient and the serums kept at -20&deg;C until use. Each sample was examined by these methods in duplicate and the sensitivity, specificity, and the positive and negative predictability values were calculated. </span><br> <strong><span style="font-size:18px;"><span style="color:#ffffff;"><span style="background-color:#16a085;">Results:</span></span></span></strong><span style="font-size:16px;"> Out of 176 patients, 92 (52.3%) were males and 84 (47.7%) were females. The age of the patients was between 15 to 65 years old with an average of 18-40 years. 72.4% of patients were from rural and 27.6% from urban areas. In 24 cases (13.3%), the Rose Bengal test was positive, while with ELISA assay, 72 patients (40.9%) were positive. The sensitivity, specificity and positive and negative predictive values of Rose Bengal test were compared to IgM ELISA values that were 100, 95.6, 70.8 and 100%, and with that IgG ELISA values were 36.9, 100, 100 and 73%, respectively. </span><br> <strong><span style="font-size:18px;"><span style="color:#ffffff;"><span style="background-color:#16a085;">Conclusion:</span></span></span></strong><span style="font-size:16px;"> In the acute phase of Brucellosis, the sensitivity of the Rose Bengal test is equal to ELISA assay and is recommended for this stage. However, in the chronic phase of the disease, the sensitivity is low and Rose Bengal test is not suggested. For this stage of the disease the ELISA assay is recommended.</span></span></div> BRUCELLOSIS, SEROLOGIC DIAGNOSIS, ELISA, ROSE BENGAL 25 31 http://journal.zums.ac.ir/browse.php?a_code=A-10-1-24&slc_lang=fa&sid=1 Hamid Baghchesaraei حمید باغچه سرایی 5200319475328460070051 5200319475328460070051 Yes Abdolreza Esmailzadeh عبدالرضا اسماعیل زاده 5200319475328460070052 5200319475328460070052 No