fa اثرات آنتی پرولیفراتیوrmIL-27 بر روی رده‌ی سلول 4T1 سرطان سینه (مدل موش)، به عنوان یک کاندید در ایمونوتراپی سرطان پستان کارآزمایی بالینی Clinical Trials <p>Background and Objective: Breast cancer, assumed as a difficult -to &ndash;treat disorder across the world, is the most common malignancy among women. Over decades, significant advances in breast cancer immunotherapy and tumor immune biology have been brought about. In vitro studies of the effect of various cytokines have been conducted in breast tumor. Among Interleukins, IL-27 -a novel cytokine- is associated with specific properties. Moreover, IL-27 contributes to the Th1 induction and also acts as a pro- inflammatory cytokine. The aim of this study was to evaluate the IL-27 anti-proliferative effects on 4T1 cell line in vitro. Materials and Methods: In this study, 4T1 cells were cultured in RPMI1640. mIL-27 gene was cloned, cells were transfected and recombinant protein was produced. Then, anti-proliferative effect of IL-27 on 4T1 breast cancer cell line was evaluated. Results: Our results indicated that IL-27 could suppress 4T1 cell proliferation significantly (p<0/01). Cell to cell interactions and also morphology of the cells were remarkably changed in comparison to control cells. Conclusion: Our results showed that, IL-27 under in vitro conditions, could potentially suppress tumor without any essential cells and biologic factors of tumor matrix. Therefore, rmIL-27 may be a probable candidate protein as an antitumor agent, applicable to breast cancer immunotherapy.</p> Keywords: Recombinant murine, IL-27, Antiproliferative, Breast tumor, Immune therapy 52 60 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-772&slc_lang=fa&sid=1 Abdoreza Esmaeilzadeh عبدالرضا اسماعیل‌زاده 5200319475328460059343 5200319475328460059343 No Dept. of Immunology, Faculty of Medical Sciences, Tarbiat Modares University ,Tehran, Iran Masoumeh Ebtekar معصومه ابتکار ebtekarm@modares.ac.ir 5200319475328460059344 5200319475328460059344 Yes Dept. of Immunology, Faculty of Medical Sciences, Tarbiat Modares University ,Tehran, Iran Alireza Biglari علیرضا بیگلری 5200319475328460059345 5200319475328460059345 No Dept. of Genetic and Molecular Medicine, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran zuhair Saraf زهیر صراف 5200319475328460059346 5200319475328460059346 No Dept. of Immunology, Faculty of Medical Sciences, Tarbiat Modares University ,Tehran, Iran Takoyaki yoshimoto تاکویوکی یوشیموتو 5200319475328460059347 5200319475328460059347 No Intractable Immune System Disease Research Center, Tokyo Medical University, Tokyo, Japan