fa بررسی جهش‌های اگزون‌های 1 و 2 ناحیه 3´UTR ژن VSX1 در بیماران مبتلا به ورم بهاره ملتحمه‌ی چشم در شهرکرد با استفاده از روش‌های PCR-SSCP/HA و Sequencing کارآزمایی بالینی Clinical Trials <p>Background and Objective: Vernal Keratoconjuctivis is an immune response in relation to environmental antigens, leading to inflammation of the conjunctiva. One of the presumable genetic factors in VKC is VSX1 gene. In this study, mutations in exon 1, exon 2 and 3&#39;UTR of VSX1 gene in patients with VKC in Shahrekord were investigated by PCR-SSCP and PCR-HA.</p> <p>Materials and Methods: In this cross- sectional study, peripheral blood samples of 100 patients with VKC and 100 individuals with no confirmed eye disease as control group were investigated. Genomic DNA was extracted by phenol-chloroform method and then PCR was carried out. Then, SSCP and HA were performed and the samples with shifted bands were sequenced for the type of nucleotide change. Afterwards, to investigate the observed nucleotide change, RFLP method was used.</p> <p>Results: Our SSCP findings revealed six patients with shifted band in exons 1 and 2 and 13 patients in 3&#39;UTR, which were sequenced for nucleotide change. Analysis of sequencing data showed a frameshift change (g. 25057561delG) in 3&#39;UTR. There was no change in other sequences.</p> <p>Conclusion: The findings of this study showed that, VSX1 gene most probably has no effective role in VKC pathogenesis in the studied population. Therefore, the role of VSX1 genes in VKC pathogens needs further investigation.</p> <p></p> Keywords: Mutation, Vernal Keratoconjuctivis, VSX1 gene 13 21 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-887&slc_lang=fa&sid=1 Elaheh Fallahi الهه فلاحی 5200319475328460058863 5200319475328460058863 No Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran علوم پزشکی شهرکرد Shahr banoo Parchami Barjui شهربانو پرچمی برجوئی 5200319475328460058864 5200319475328460058864 No Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran علوم پزشکی شهرکرد Sumayyeh Reiisi سمیه رئیسی 5200319475328460058865 5200319475328460058865 No National Institute for Genetic Engineering and Biotechnology, Tehran, Iran دانشگاه تهران Ali Salehi علی صالحی 5200319475328460058866 5200319475328460058866 No Dept. of Ophthalmology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran علوم پزشکی اصفهان Beheshteh Amiri بهشته امیری 5200319475328460058867 5200319475328460058867 No Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran علوم پزشکی شهرکرد Fatemeh Heybati فاطمه هیبتی 5200319475328460058868 5200319475328460058868 No Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran علوم پزشکی شهرکرد Hosein teimori حسین تیموری hteimori@skums.ac.ir 5200319475328460058869 5200319475328460058869 Yes Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran علوم پزشکی شهرکرد