fa مقایسه اثر دو منبع نور لیزری متفاوت بر بازده درمان فتودینامیکی سرطان پستان در شرایط برون تنی کارآزمایی بالینی Clinical Trials <p dir="ltr" style="text-align: justify"><strong><em>Background and Objective:</em></strong> Photodynamic therapy is a new therapeutic modality for the treatment of cancer. Photodynamic therapy uses an inactive drug and a light source to activate the drug to produce reactive oxygen species that destroy the cancer cells. In the present study, the effect of two different laser light sources on the efficiency of photodynamic therapy was evaluated using a breast cancer cell line.</p> <p dir="ltr" style="text-align: justify"><strong><em>Materials and Methods:</em></strong> MDA-MB-231cells were grown on 96-well plates. After the treatment with different concentrations of 5-aminolevulinic acid, cells were irradiated with a light dose of 10 and/ or 20 Jcm^-2 by two different laser light sources. Cell viability was measured by MTT assay.</p> <p dir="ltr" style="text-align: justify"><strong><em>Results:</em></strong> Results showed that 5-aminolevulinic acid at concentrations of 0.5, 1 and 2 mM had no significant cytotoxic effect on the breast cancer cells in the absence of laser activation. However, the treatment of cells with 5-aminolevulinic acid and light dose of 20 Jcm^-2 using Lasotronic laser and Mustang laser, decreased cell viability of the breast cancer cells to 80% and 65%, respectively.</p> <p dir="ltr" style="text-align: justify"><strong><em>Conclusion:</em></strong> Light source used in photodynamic therapy plays an important role in the efficiency of this therapeutic modality and light activation of 5-aminolevulinic acid at a photosensitizing concentration using a proper laser light source may potentially be lethal for breast cancer cells.</p> <p dir="ltr" style="text-align: justify"></p><p dir="ltr"></p> Keywords: Photodynamic therapy, Light source, Laser, Breast cancer 1 12 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-898&slc_lang=fa&sid=1 Morvarid Tabbodi مروارید تعبدی 5200319475328460057386 5200319475328460057386 No Institute of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran. سازمان پژوهش‌های علمی و صنعتی ایران Mahnaz Hadizadeh مهناز هادی زاده Hadizadehmahnaz@gmail.com 5200319475328460057387 5200319475328460057387 Yes Institute of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran. سازمان پژوهش‌های علمی و صنعتی ایران Maryam Jahanshiri-Moghadam مریم جهانشیری مقدم 5200319475328460057388 5200319475328460057388 No Medical Laser Research Center, Academic Center for Education, Culture & Research (ACECR), Tehran, Iran. علوم پزشکی تهران