fa بررسی وضعیت آلودگی کارکنان، ادوات و مواد غذایی با سویه های استافیلوکوکوس اورئوس مقاوم به متی سیلین در یک واحد طبخ بیمارستانی کارآزمایی بالینی Clinical Trials <p dir="ltr"><strong><em>Background and Objective:</em></strong> <em>Staphylococcus aureus </em>(<em>S. aureus</em>), particularly methicillin- resistant strain (MRSA), is one of the most important pathogens in nosocomial infections that is capable of producing food poisoning and food-borne outbreaks. This study aimed to focus on the contamination status of food handlers, utensils and foodstuffs with this agent in a hospital kitchen as sources of distribution of these strains into hospitals<span dir="RTL">.</span></p> <p dir="ltr"><strong><em>Materials and Methods:</em></strong> A total of 220 samples were collected from freshly prepared foods, kitchen utensils and food handlers. The samples were cultured in Blood agar and Mannitol salt agar media and the isolates were evaluated both morphologically and biochemically using specialized tests and consequently were identified via polymerase chain reaction (PCR) using <em>femA</em> and <em>nuc </em>primers. For antibiogram, the standard disk diffusion test was used in accordance with CLSI, 2012 guidelines. To detect MRSA strains, resistance of the<em> S. aureus </em>isolates to cefoxitin (30 &micro;g) in Mueller-Hinton agar was evaluated.</p> <p dir="ltr"><strong><em>Results: </em></strong>Thirty- nine S. aureus isolates from food handlers (50%), 26 from kitchen utensils (48.1%), and 22 from food samples (25%) were detected. MRSA strains were found in 22.7% of the S. aureus isolates of foodstuffs, 23.1% in utensils and 5.1% in food handler samples. Homology of the resistance patterns among these isolates was also observed.</p> <p dir="ltr"><strong><em>Conclusion:</em></strong> This study showed the presence of MRSA strains and homology of resistance patterns among the isolates of the foodstuffs, kitchen utensils and food handlers all of which call for a careful monitoring to prevent their distribution among hospitalized patients.</p> <p></p> Keywords: Methicillin-resistant Staphylococcus aureus, Hospital food, Utensils, Food handlers 24 35 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-900&slc_lang=fa&sid=1 Gohar taj Rassouli گوهر تاج رسولی 5200319475328460058829 5200319475328460058829 No Dept of Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. دانشگاه علوم پزشکی زنجان Masoud Alebouyeh مسعود آل بویه Masoud.alebouyeh@gmail.com 5200319475328460058830 5200319475328460058830 Yes Foodborne and Waterborne Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. دانشگاه علوم پزشکی شهید بهشتی Bahram Amini بهرام امینی 5200319475328460058831 5200319475328460058831 No Dept of Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. دانشگاه علوم پزشکی زنجان Ali Rassouli علی رسولی 5200319475328460058832 5200319475328460058832 No Dept of Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. دانشگاه تهران Moammad Reza Zali محمد رضا زالی 5200319475328460058833 5200319475328460058833 No Gasteroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. دانشگاه علوم پزشکی شهید بهشتی