fa بررسی ژن‌های اگزاسیلیناز در ایزوله‌های بالینی اسینتوباکتربامانی مقاوم به کرباپنم در بیمارستان شهید محمدی بندرعباس درسال 1393 کارآزمایی بالینی Clinical Trials <p dir="ltr" style="text-align: justify"><strong><em>Background and Objective</em></strong><strong>:</strong> Acinetobacter baumannii is considered as an important agent in nosocomial infections. The problem facing the tratment of these bacteria is increasing resistance to antimicrobial agents such as carbapenems. The main mechanism of resistance to carbapenems in these bacteria is the presence of&nbsp; oxacillinases genes, which belong to class D betalactamases. The aim of this study was &nbsp;&nbsp;molecular diagnosis of Acinetobacter baumannii and&nbsp; antimicrobial resistance pattern of these bacteria to antibiotics including imipenem and meropenem.&nbsp;</p> <p dir="ltr" style="text-align: justify"><strong><em>Materials and Methods</em></strong><strong>:</strong> Genus and species of clinical isolates of <em>Acinetobacter</em> were identified via biochemical tests and PCR method respectively. Their susceptibily&nbsp; to imipenem and meropenem&nbsp; were determined by disk diffusion method.&nbsp; In order to identify carbapenem resistant genes (oxacillinases), multiplex PCR was used.</p> <p dir="ltr" style="text-align: justify"><strong><em>Results:</em></strong> 76 of 72 (94.7%) <em>Acinetobacter</em> species, possessed <em>bla</em> <em>_oxa-51-</em>like gene and were identified as&nbsp; <em>A.baumannii</em>. Sixty one (84.7%) of isolates were resistant to imipenem and 70(97.2% ) were resistant to meropenem. Out of 76 isolates, 63 (87.5%) had an acquired <em>bla</em> _oxa-23like carbapenemase, 7 (9.7%%) possessed <em>bla</em> _oxa-24-like carbapenemase. None of the isolats contained either <em>bla</em> <em>_oxa-58</em> or <em>bla</em> <em>_oxa-143</em> carbapenemase.</p> <p dir="ltr" style="text-align: justify"><strong><em>Conclusions</em></strong><strong>:</strong> It appears that cabapenem overutilization causes a selective pressure resulting in the emergence of antibiotic- resistant isolates. This study indicated that, the presence of <em>bla </em>_oxa-23 genes was significantly associated with the occurrence of resistance to carbapenems. So, investigation of antibiotic resistance genes in <em>A. baumannii</em> is necessary to control further dissemination of these resistance genes.</p> <p dir="ltr" style="text-align: justify"></p><p></p><p dir="ltr" style="text-align: justify"></p><p></p><p dir="ltr" style="text-align: justify"></p> Keywords: Acinetobacter baumannii, Carbapenemase, Antibiotic resistant 45 54 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-902&slc_lang=fa&sid=1 Fahimeh Bahadori azimabadi فهیمه بهادری عظیم ابادی 5200319475328460058838 5200319475328460058838 No Dept .of Microbiology,Faculty of Science, Azad University, Shiraz, Iran دانشگاه آزاد شیراز Afsaneh Karmostaji افسانه کرمستجی Afsanehkk@yahoo.com 5200319475328460058839 5200319475328460058839 Yes Infectious and Tropical Diseases Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran دانشگاه علوم پزشکی هرمزگان