fa بررسی اثر پیش درمان اریتروپویتین بر محتوای آب بافتی مغز به دنبال ایسکمی مغزی در موش صحرایی نر با مدل انسداد شریان مغزی- میانی کارآزمایی بالینی Clinical Trials <pre> <strong><em>Background and Objective: </em></strong>One of the major side effects of cerebral ischemia is the water content enhancement in brain tissues which leads to mortality enhancement. Recently, therapeutic strategies have been focusing on preconditioning mechanisms. Erythropoietin as a glycoprotein hormone with anti-inflammatory and antioxidant effects on brain may be effective on brain edema reduction.</pre> <pre> <strong><em>Materials and Methods: </em></strong>Male wistar rats were assigned to 5 groups of 6 in each: sham, control and three pretreatments. The pretreatment groups including single-dose (0.5 h before stroke induction), double-dose (0.5 and 48 h before stroke induction) and triple-dose (0.5, 48 and 98 h before stroke induction) received 1,000 IU/kg EPO intraperitoneally. 0.5 hour after the last injection, the animals were exposed to brain ischemia with the MCAO model for 60 minutes and then were evaluated regarding the brain water content, hemoglobin and hematocrit at 24 hours after reperfusion.</pre> <pre> <strong><em>Results:</em></strong> Brain water content in the pretreatment group receiving EPO with a lower dose (1000 U/kg) decreased significantly but in the pretreatment group with a higher dose of the drug a significant enhancement was observed. Also, the amount of hemoglobin and hematocrit increased in the pretreatment groups in comparison with the control group.</pre> <pre> <strong><em>Conclusion:</em></strong> Our results showed single-dose of EPO pretreatment decreased brain edema in the brain ischemic stroke model in wistar rats.</pre> <pre> </pre> <pre> </pre> Keywords: Erythropoietin, Pretreatment, Brain ischemia, Brain water content, Middle Cerebral Artery Occlusion 59 72 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-941&slc_lang=fa&sid=1 Mohammadreza Bigdehi محمدرضا بیگدلی 5200319475328460053755 5200319475328460053755 No Dept. of Physiology, Fculty of Life Sciences, Shahid Beheshti University, Tehran, Iran دانشگاه شهید بهشتی تهران Hossein Mostafavi حسین مصطفوی 5200319475328460053756 5200319475328460053756 No Dept. of Physiology and Pharmacology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشگاه علوم پزشکی زنجان Raheleh Gholamzadeh راهله غلام‌زاده 5200319475328460053757 5200319475328460053757 No Dept. of Physiology and Pharmacology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشگاه علوم پزشکی زنجان Mahdi Eskandari مهدی اسکندری mehdiesk@zums.ac.ir 5200319475328460053758 5200319475328460053758 Yes Dept. of Physiology and Pharmacology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشگاه علوم پزشکی زنجان