fa ررسی ارتباط پلی‌مورفیسم تک نوکلئوتیدی rs 10853728 در ناحیه‌ی پروموتر ژن IL-28B در بیماران ایرانی مبتلا به هپاتیت C تحت درمان با داروهای پگ اینترفرون آلفا و ریباورین کارآزمایی بالینی Clinical Trials <p dir="LTR"><strong><em>Background and Objective:</em></strong> Genetic factors greatly impact the response to treatment in patients. Recent studies on rs10853728 single nucleotide polymorphisms in the promoter area, determined the IL-28B gene as a host factor affecting the treatment of hepatitis C infection. The aim of this study was to evaluate this polymorphism among Iranian patients</p> <p dir="LTR"><strong><em>Materials and Methods:</em></strong> This cross-sectional study was performed on 53 blood samples of patients with hepatitis C (49 patients were sensitive and 4 patients resistant to treatment) and 30 healthy controls. After DNA extraction from buffy coat samples, the frequency of polymorphisms was determined. Finally, the PCR products were detected on agarose gel electrophoresis. For statistical analysis of the data, the chi-square method was used.</p> <p dir="LTR"><strong><em>Results:</em></strong> About 30 healthy controls (negative for HCV Ab ELISA test) participated in this survey. Of the 53 patients tested, none of them were homozygous CC (Wild Type) and only 2 (%3.8) were homozygous GG, one of which was sensitive to treatment. Of the 51 remaining patients, 48 (%96.2) were heterozygous CG and sensitive to treatment with peg interferon and ribavirin while 3 of the patients were resistant to treatment.</p> <p dir="LTR"><strong><em>Conclusion: </em></strong>Statistical analysis showed that patients with the G allele had significantly higher sustained viralogic response (SVR) rate than those with the C allele. This data suggest that genotype detection of rs10853728&nbsp; single nucleotide polymorphism may be useful as an important predictive biomarker for SVR in patients infected with HCV. However, further studies with more samples will lead to more valid results.</p> <p dir="LTR"></p><p dir="LTR"></p> Keywords: Hepatitis C, Interleukin 28B, Polymorphism 31 42 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-986&slc_lang=fa&sid=1 Mahsa Zamanian مهسا زمانیان 5200319475328460053613 5200319475328460053613 No Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran دانشگاه علوم پزشکی زنجان Saeideh Soleimani سعیده سلیمانی 5200319475328460053614 5200319475328460053614 No Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran دانشگاه آزاد واحد تهران Zahra Paz زهرا پاز 5200319475328460053615 5200319475328460053615 No Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran دانشگاه آزاد واحد تنکابن Fereshteh Ferdosian فرشته فردوسیان 5200319475328460053616 5200319475328460053616 No Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran دانشگاه تربیت مدرس Golnaz Asaadi Tehrani گلناز اسعدی طهرانی 5200319475328460053617 5200319475328460053617 No Dept. of Genetics, Islamic Azad University, Zanjan Branch, Zanjan, Iran دانشگاه آزاد اسلامی واحد زنجان Farahnaz Bineshian فرحناز بینشیان 5200319475328460053618 5200319475328460053618 No Dept. of Mycology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran دانشگاه علوم پزشکی سمنان Zohreh Sharifi زهره شریفی 5200319475328460053619 5200319475328460053619 Yes Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran انتقال خون تهران