fa بررسی فراوانی موتاسیون آللی CCR5Δ32 در افراد سالم استان زنجان کارآزمایی بالینی Clinical Trials <p><strong><em>Background and Objective: </em></strong>CCR5 is known as a main co-receptor in HIV infection. Many studies demonstrated that 32bp deletion in both <em>CCR5</em> alleles provide natural resistance to HIV infection, so new treatment approaches are based on inducing this resistance by means of gene and cell therapies. This projects aim was the discovery of suitable candidates for new therapeutic strategies in addition to determining the <em>CCR5 &Delta;32</em> mutation frequency in healthy individuals in Zanjan province for the first time.</p> <p><strong><em>Materials and Methods:</em></strong> Blood samples were collected from 200 healthy individuals in EDTA pre-coated tubes. Genomic DNA was extracted and analyzed for <em>CCR5 &Delta;32</em> mutation by GAP-PCR. The allele frequency was calculated using the Hardy-Weinberg equilibrium (HWE).</p> <p><strong><em>Results:</em></strong> Our findings showed 1 healthy individual had <em>CCR5 &Delta;32/&Delta;32</em> mutation and the allele frequency of <em>CCR5&Delta;32</em> was calculated to be 0.5%.</p> <p><strong><em>Conclusion:</em></strong> Results of this study proved previous reports that Iranian populations are more susceptible to HIV/AIDS compared to the European population. Different climate-geographical conditions, migration and positive natural selection could explain various distribution of <em>&Delta;32</em> allele frequency in European countries compared to Iran. Consequently, Iranians require more effective and novel therapeutic approaches for HIV infection therapy and could benefit from reducing retro-viral drug restrictions.</p> <p></p><p></p> Key Words: CCR5 Δ32, HIV, AIDS, Iran 129 136 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-992&slc_lang=fa&sid=1 Aliyeh Farshbaf عالیه فرشباف 5200319475328460053658 5200319475328460053658 No Dept. of Genetic & Molecular Medicine, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشگاه علوم پزشکی زنجان Alireza Biglari علیرضا بیگلری 5200319475328460053659 5200319475328460053659 No Dept. of Genetic & Molecular Medicine, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشگاه علوم پزشکی زنجان Soghrat Faghihzadeh سقراط فقیه‌زاده 5200319475328460053660 5200319475328460053660 No Dept. of Biostatistics and Epidemiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشگاه علوم پزشکی زنجان Abdolreza Esmaeilzadeh عبدالرضا اسماعیل‌زاده a46reza@zums.ac.ir 5200319475328460053661 5200319475328460053661 Yes Dept. of Immunology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشگاه علوم پزشکی زنجان