fa بررسی کاهش نرخ آپوپتوز توسط والپروئیک اسید به عنوان یک فعال کننده‌ی STAT5 در بیماران مبتلا به آتروفی عضلانی نخاعی کارآزمایی بالینی Clinical Trials <p><strong><em>Background and Objective:</em></strong> The severity of the spinal muscular atrophy phenotype is inversely associated with the expression levels of the SMN2 gene; this correlation is not absolute, for this reason there is currently no effective treatment. The interference of other severity modifying factors, apart from SMN2 gene expression has been suggested. Here we investigate the effects of valproic acid as a STAT5 inducer not only on SMN2 expression but on STAT5A gene expression as well as on the rate of apoptosis.</p> <p><strong><em>Materials and Methods: </em></strong>Expression levels of the SMN2 and STAT5A genes were determined using Real-time PCR while apoptotic rates were determined using flow cytometry in lymphocyte cell lines derived from three patients and two normal controls in response to valproic acid.</p> <p><strong><em>Results:</em></strong> STAT5A gene induction, enhanced FL-SMN2 expression and reduced apoptotic rate were seen in lymphocyte cell lines after valproic acid treatment (p<0.05, t-test).</p> <p><strong><em>Conclusion:</em></strong> Since STAT5A gene induction was accompanied with SMN2 transcriptional upregulation, these findings propose the involvement of STAT5A gene both as a component of the STAT5 pathway and as a target gene in drug responsiveness.</p> <p></p><p></p> Keywords: Spinal Muscular Atrophy, Valproic acid, Real-time PCR, Apoptotic rate 56 67 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-994&slc_lang=fa&sid=1 ْGholamreza Bahrami Monajemi غلامرضا بهرامی منجمی 5200319475328460053639 5200319475328460053639 No Dept. of Medical Genetics, Faculty of Medicine, Tarbiat Modares University, Tehran Iran دانشگاه تربیت مدرس Mehrdad Noruzinia مهرداد نوروزی نیا 5200319475328460053640 5200319475328460053640 Yes Dept. of Medical Genetics, Faculty of Medicine, Tarbiat Modares University, Tehran Iran دانشگاه تربیت مدرس Sayed Hassan Tonekaboni سیدحسن تنکابنی 5200319475328460053641 5200319475328460053641 No Dept. of Neurology, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی شهید بهشتی تهران