fa تاثیر محافظتی کروسین بر عملکرد کلیه در نفروپاتی ناشی از هیپرگلیسمی مزمن کنترل نشده در موش صحرایی کارآزمایی بالینی Clinical Trials <p><strong><em>Background and Objective</em></strong><strong>:</strong> Based on previous studies, inflammation and oxidative stress play a crucial role in the pathophysiology of diabetic nephropathy. Therefore, we aimed to examine the protective effects of crocin, as a potent anti-inflammatory and antioxidant, on kidney function and oxidative stress in an experimental model of diabetic nephropathy in male wistar rats.</p> <p><strong><em>Materials and Methods</em></strong><strong>:</strong> In this experimental study, male wistar rats (190-220 g) were randomly divided into four groups (each n=6); normal, normal treatment, diabetic, diabetic treatment. The rats were made diabetic by an intravenous injection of streptozotocin (40 mg/kg). Treated rats received crocin intraperitoneally with a dose of 40 mg/kg/day for eight weeks. Blood samples were collected for measurement of blood glucose and creatinine levels. The Malondialdehyde (MDA) and nitrate contents of kidney also were assessed.</p> <p><strong><em>Results</em></strong><strong>:</strong> Chronic hyperglycemia (blood glucose >400 mg/dL) significantly increased the blood creatinine of the diabetic group (4.51&plusmn;0.45 mg/dL) compared with normal rats (0.72&plusmn;0.09 mg/dL, P<0.001). Hyperglycemia also decreased the nitrate content (37%) accompanied by an increase in the MDA content (13.00&plusmn;0.19 &micro;g/mg protein) compared with normal group (10.22&plusmn;0.54 &micro;g/mg protein). Crocin significantly decreased the blood creatinine of diabetic rats (1.12&plusmn;0.13 mg/dL, P<0.001), accompanied by a decrease in blood glucose (9%). Finally, the content of MDA significantly decreased by 29% in diabetic treated rats compare to diabetic untreated rats (P<0.001)<span dir="RTL">.</span></p> <p><strong><em>Conclusion:</em></strong> Based on the findings of the present study, it is appeared that crocin has a role in the prevention of diabetic nephropathy through inhibition of oxidative stress and reducing lipid oxidation.</p> <p></p><p></p> Key words: Diabetes Mellitus, Crocin, Diabetic Nephropathy, Oxidative Stress, Hyperglycemia 36 49 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-998&slc_lang=fa&sid=1 Habib Yaribeygi حبیب یاری بیگی 5200319475328460053397 5200319475328460053397 No Dept. of Physiology and Biophysics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی بقیه الله تهران Mohammad Taghi Mohammadi محمد تقی محمدی Mohammadi.mohammadt@yahoo.com 5200319475328460053398 5200319475328460053398 Yes Dept. of Physiology and Biophysics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی بقیه الله تهران