fa بررسی اثر عصاره‌ی روغنی بذر سیاه دانه (N. sativa) بر بقا، بیان ژنVEGF-A و آپوپتوز در رده‌ی سلولی سرطانی معده AGS(CRL-1739) کارآزمایی بالینی Clinical Trials <pre> <a><strong><em>Background</em></strong></a><a href="#_msocom_1" id="_anchor_1" name="_msoanchor_1">[d1]</a>&nbsp;<strong><em> and Objective</em></strong><strong><em>:</em></strong> Cancer is a prevalent disease that occurs due to genetic and environmental factors. Gastric cancer is one of the most common cancers around the world and also in Iran. N. sativa has been used as a drug in the treatment of many diseases owing to its valuable secondary metabolites such as <em>t</em><em>hymoquinone</em>. &nbsp;In this study, the anti-cancer properties of black seed extract on gastric cancer cells was studied and the seeds oil was evaluated for possible drug use.</pre> <p><strong><em>Materials and Methods:</em></strong> Stomach gastric adenocarcinoma (AGS) cell line was cultured and treated with various concentrations of <em>N. sativa</em> seed soil for 24 hours. Consequently the cytotoxic effect of the extract on the cell line was determined using MTT assay while the rate of apoptosis was determined by Annexin V and Flow cytometry and finally, VEGF-A expression was determined by Real-Time PCR.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</p> <p><strong><em>Results:</em></strong> Black seed oil treatment significantly reduced cancer cell proliferation compared to control cells. This treatment also led to an increased rate of programmed cell death. However, Black seed oil treatment had no effect on the expression of VEGF-A.&nbsp;&nbsp;</p> <pre> <strong><em>Conclusion: </em></strong>Extract of black seed has a positive impact on reducing proliferation and elevating apoptosis in cancer cells.</pre> <p></p><p></p> <div> <hr align="left" size="1" width="33%" > <div> <div id="_com_1"><a name="_msocom_1"></a> <p></p></div> </div> </div> AGS cell line, Stomach Cancer, Black seed, Angiogenesis, Apoptosis 15 28 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1020&slc_lang=fa&sid=1 Mohsen Rashid Shyekh Ahmad محسن رشید شیخ احمد 5200319475328460053248 5200319475328460053248 Yes National Institute of Genetic Engineering and Biotechnology, Department of Agricultural Biotechnology, Tehran, Iran پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران Farzaneh Sabouni فرزانه صابونی 5200319475328460053249 5200319475328460053249 No National Institute of Genetic Engineering and Biotechnology, Department of Agricultural Biotechnology, Tehran, Iran پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران Forough Sanjarian فروغ سنجریان 5200319475328460053250 5200319475328460053250 No National Institute of Genetic Engineering and Biotechnology, Department of Agricultural Biotechnology, Tehran, Iran پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران