fa ارزیابی اثرات محیط کشت رویی پرده آمنیون بر فعالیتHeat Shock Protein 90 در سلول‌های سرطانی سرویکس و پستان مقاله پژوهشی Original Research Article <p><strong><em>Background and Objective: </em></strong>It has recently been shown that the application of amniotic membrane conditioned medium is effective in cancer treatment. In this study, the effect of amniotic stem cells conditioned medium on the activity of Hsp90 and Cdk4 expression, were investigated in cancer cells.</p> <p><strong><em>Materials and Methods: </em></strong>Two cancer cell lines HeLa and MDA-MB-231 were treated with the supernatant amniotic membrane conditioned medium for 24 hours and then the viability of cancer cells was evaluated by MTT assay. Immunocytochemistry assay was used to investigate changes in Cdk4 expression in cancer cells after being treated with the amniotic membrane conditioned medium. RT-PCR analysis was carried out to study the expression of Hsp90&alpha; (aa1) gene before and after treatment.</p> <p><strong><em>Results:</em></strong> The results from the MTT assay indicated that the viability of cancer cells decreased after treatment with amniotic membrane conditioned medium. The outcomes of immunocytochemistry showed a decrease in Cdk4 expression in treated cancer cells. Evaluation of Hsp90&alpha; (aa1) expression with RT-PCR analysis for cancer cells treated with amniotic membrane conditioned medium revealed a reduction in the expression of the Hsp90&alpha; (aa1) gene.</p> <p><strong><em>Conclusion:</em></strong> Based on the results, treatment of cancer cells with amniotic membrane conditioned medium, in addition to the reduction of cancer cell viability, leads to the induction of apoptosis through the inhibition of Hsp90 and Cdk4. According to the results, it seems that the inhibition of Hsp90 could be the possible mechanism behind the anticancer properties of amniotic membrane.</p> <p></p><p></p> Keywords: Amniotic membrane, Apoptosis, Hsp90, Cancer 10 20 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1032&slc_lang=fa&sid=1 Masoumeh Mirmasoumi معصومه میر معصومی 5200319475328460053165 5200319475328460053165 Yes Dept. of Pharmacology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشکده‌ی پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران Sara Azizian سارا عزیزیان 5200319475328460053166 5200319475328460053166 No Dept. of Biomaterials, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran دانشکده‌ی مهندسی پزشکی، دانشگاه صنعتی امیرکبیر، تهران Khashayar Modaresifar خشایار مدرسی فر 5200319475328460053167 5200319475328460053167 No Dept. of Biomaterials, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran دانشکده‌ی مهندسی پزشکی، دانشگاه صنعتی امیرکبیر، تهران Hamideh Moravvej حمیده مروج 5200319475328460053168 5200319475328460053168 No Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشگاه علوم پزشکی شهید بهشتی، تهران Hasan Niknejad حسن نیک نژاد niknejad@sbmu.ac.ir 5200319475328460053169 5200319475328460053169 No Dept. of Pharmacology Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran دانشکده ی پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران