fa ارزیابی میزان بیان ژن GRAF در بیماران B-ALL مراجعه کننده به بیمارستان‌های مشهد و ارتباط آن با یافته‌های آزمایشگاهی و بالینی بیماران کارآزمایی بالینی Clinical Trials <p style="text-align: justify;"><strong><em>Background and Objective:</em></strong> Acute lymphoblastic leukemia is a disorder caused by the proliferation of malignant immature lymphoid cells. GRAF (GTPase regulator associated with focal adhesion kinase) which acts as a tumor suppressor gene is disabled in hematologic malignancies due to genetic and epigenetic abnormalities. In this study, GRAF gene expression was inspected in patients with B-ALL.</p> <p style="text-align: justify;"><strong><em>Materials and Methods:</em></strong> Peripheral blood samples from 60 patients with B-ALL and 30 healthy controls were collected. DNA was extracted and cDNA was synthesized. GRAF gene expression was measured using Real-Time PCR and the relationship between GRAF expression and clinical and laboratory findings were investigated.</p> <p style="text-align: justify;"><strong><em>Results:</em></strong> GRAF gene expression was significantly decreased in patients compared to the control group (0.87).<br> The number of patients who had a reduction in their GRAF gene expression was 73.4% (44 of 60 patients). GRAF expression had no significant difference in the FAB morphological subtypes.</p> <p style="text-align: justify;"><strong><em>Conclusion:</em></strong> Results of this study showed that GRAF gene expression is decreased in acute lymphoblastic leukemia. The reduction in GRAF gene expression confirms its tumor suppressive role in this leukemia.</p> <p></p><p></p><p dir="RTL"></p><p></p> Key words: GRAF, Acute Leukemia, Real-Time PCR, ALL 40 49 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1034&slc_lang=fa&sid=1 Abbas Ghotaslou عباس قوطاسلو 5200319475328460053181 5200319475328460053181 No Student Research Committee, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran دانشکده‌ی پیراپزشکی، دانشگاه علوم پزشکی ایران، تهران Hasan Boustani حسن بوستانی 5200319475328460053182 5200319475328460053182 No Student Research Committee, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran دانشکده‌ی پیراپزشکی، دانشگاه علوم پزشکی ایران، تهران Esmaeil Rostami اسماعیل رستمی 5200319475328460053183 5200319475328460053183 Yes Dept. of Laboratory Sciences, Faculty of Para- Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran دانشکده‌ی پیراپزشکی، دانشگاه علوم پزشکی سبزوار، سبزوار Omid Ghalesardi امید کیانی قلعه سردی 5200319475328460053184 5200319475328460053184 No Student Research Committee, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran دانشکده‌ی پیراپزشکی، دانشگاه علوم پزشکی ایران، تهران Mohammad Hadi Sadeghian محمد هادی صادقیان 5200319475328460053185 5200319475328460053185 No Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran دانشگاه علوم پزشکی مشهد، مشهد