fa بررسی اثر نوروپروتکتیو آدنوزین بر بقای سلول‌های بنیادی عصبی در شرایط آسیب اکسیداتیو با پراکسید هیدروژن کارآزمایی بالینی Clinical Trials <p><strong><em>Background and Objective: </em></strong>Excessive production of free radicals during oxidative stress can cause serious damage to important biomolecules and activate programmed cell death pathways in the body. In the nervous system, neuronal cell death leads to many progressive neurodegenerative disorders. The aim of this study was to evaluate the effects of adenosine on the inhibition of apoptosis in oxidative damage induced by hydrogen peroxide in bone marrow derived neural stem cells (BMSCs-dNSCs)<span dir="RTL">. </span></p> <p><strong><em>Materials and Methods:</em></strong> BMSCs-dNSCs were pretreated with different doses of adenosine for 48 hours and then exposed to 125&mu;M H2O2 for 30 minutes. Using MTT and TUNEL assay, we evaluated the effects of adenosine on cell survival and apoptosis in pretreated BMSCs-dNSCs in comparison to control groups.</p> <p><strong><em>Results: </em></strong>The results showed that the apoptosis rate in the 6 <span dir="RTL">&micro;</span>M adenosine pretreated BMSCs-dNSCs was significantly decreased compared to the control group in the condition of oxidative stress (P<0.05). &nbsp;</p> <p><strong><em>Conclusion: </em></strong>Our findings suggest that adenosine protects NSCs against oxidative stress induced cell death, therefore it may be used to promote the survival rate of NSCs and could be a candidate for the treatment of oxidative stress mediated neurological diseases.</p> <p></p><p></p> Keywords: Adenosine, Neural stem cells, Apoptosis 72 85 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1041&slc_lang=fa&sid=1 Masoumeh Gholinejad معصومه قلی ن‍‍ژاد 5200319475328460053192 5200319475328460053192 Yes Dept. of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان Ali Reza Abdanipour علیرضا عبدانی پور 5200319475328460053193 5200319475328460053193 No Dept. of Anatomy, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان Amir Hossein Taromchi امیر حسین تارمچی 5200319475328460053194 5200319475328460053194 No Dept. of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان Iraj Jafari Anar Kooli ایرج جعفری انارکولی 5200319475328460053195 5200319475328460053195 No Dept. of Anatomy, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان Ali Nikfar علی نیک فر 5200319475328460053196 5200319475328460053196 No Dept. of Genetics and Molecular Medicine, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان