fa اثر ضد آپوپتوزی گرلین در سلول‌های بنیادی استرومایی مشتق از مغز استخوان موش صحرایی تیمار شده با پراکسید هیدروژن کارآزمایی بالینی Clinical Trials <strong><em>Background and Objective: </em></strong>Apoptosis is a form of programmed cell death and oxidative stress is one of the factors that lead to apoptosis. Ghrelin is a 28 amino acid peptide and its antiapoptotic effects have been shown previously. The aim of this study was to evaluate the effect of ghrelin on cell survival and apoptosis of rat bone marrow mesenchymal stem cells (BMSCs).<br> <strong><em>Materials and Methods: </em></strong>BMSCs were cultured as control and H2O2 treated groups with and without different doses of ghrelin (0.1-1-10-100 &micro;M) for 24 and 48 hours. To find out the best dose and time of effect for ghrelin, MTT was used to determine cell survival. BMSC apoptosis rate was detected using the TUNEL technique.<br> <strong><em>Results: </em></strong>Cultured BMSCs showed that the cells are fibroblastic in appearance and adherent to culture dishes as well as being capable of having a very high proliferation rate. Ghrelin treatment increased proliferation and cell survival in both normal and H₂O₂ exposed BMSCs. The best dose and time that ghrelin could affect cell proliferation was 100&micro;M and 48 hours respectively. Treatment with ghrelin significantly reduced apoptosis in BMSCs in this dose and time.<br> <strong><em>Conclusion: </em></strong>Ghrelin treatment causes an increase in BMSCs proliferation and reduction of H2O2 induced apoptosis in these cells.<br> &nbsp;<br> &nbsp; Keywords: Apoptosis, Ghrelin, H2O2, BMSC, Rat 58 68 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1047&slc_lang=fa&sid=1 Alireza Abdanipour علیرضا عبدانی پور 5200319475328460052650 5200319475328460052650 No Dept. of Anatomical Sciences, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دکترای تخصصی علوم تشریح، استادیار گروه علوم تشریح، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان Behnaz Shahsavandi بهناز شاهسوندی 5200319475328460052651 5200319475328460052651 No Dept. of Physiology and Pharmacology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشجوی کارشناسی ارشد گروه فیزیولوژی و فارماکولوژی دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان Masoud Dadkhah مسعود دادخواه 5200319475328460052652 5200319475328460052652 No Dept. of Physiology and Pharmacology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دکترای تخصصی فیزیولوژی، استاد گروه فیزیولوژی و فارماکولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان Mohsen Alipour محسن علیپور 5200319475328460052653 5200319475328460052653 No Dept. of Physiology and Pharmacology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دکترای تخصصی فیزیولوژی، استادیار گروه فیزیولوژی و فارماکولوژی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان Hadi Feizi هادی فیضی hfeizik@zums.ac.ir 5200319475328460052654 5200319475328460052654 Yes Dept. of Physiology and Pharmacology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دانشکده‌ی پزشکی، دانشگاه علوم پزشکی زنجان، زنجان