fa بارگذاری آنتی بیوتیک سفتازیدیم بر روی اتیلن دی آمین تترا استیک اسید: بررسی سینتیک رهایش دارو و افزایش اثر ضدمیکروبی آن بر روی سودوموناس آئروژینوزا کارآزمایی بالینی Clinical Trials <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#008080;"><strong><em>Background and Objective:</em></strong></span><em> Pseudomonas aeruginosa</em> is one of the most important causes of nosocomial infections. This study was planned to produce a new antimicrobial complex (ceftazidime loaded EDTA) and assess its release kinetic and antimicrobial effects on <em>P. aeruginosa. </em><br> <span style="color:#008080;"><strong><em>Materials and Methods:</em></strong></span> The loading of ceftazidime on EDTA was performed and confirmed by FT-IR spectroscopy. The evaluation of drug release was performed by using dialyze method at 0.5, 1, 2, 3, 4, 6, 8, 12, 24, 48, 72 and 96 h after ceftazidime loading on EDTA. The antimicrobial effect of the new drug on <em>P. aeruginosa</em> was investigated by macrodilution and well diffusion methods.<br> <span style="color:#008080;"><strong><em>Results:</em></strong></span> FT-IR spectroscopy demonstrated ceftazidime loading on EDTA about 26.01 mol%. Evaluation of ceftazidime release indicated a gradual rise in drug release up to 8 h reaching 96% after 72 h. Well diffusion assay demonstrated an enhanced antimicrobial effect of ceftazidime after loading on EDTA in comparison with ceftazidime alone. MIC and MBC of ceftazidime alone determined 2 and 4 &micro;g/ml, respectively.&nbsp; MIC of the new antimicrobial complex was 0.5 &micro;g/ml and its MBC was 1 &micro;g/ml signifying the enhancement effect of EDTA on antibacterial activity of ceftazidime.<br> <span style="color:#008080;"><strong><em>Conclusion:</em></strong></span> Our results showed that EDTA is potentially advantageous in controlling the release of ceftazidime as well as enhancing its antimicrobial effect against <em>P. aeruginosa</em>.</span></span></p> Pseudomonas aeruginosa, Ceftazidime, EDTA, Controlled release, Antimicrobial effect 60 70 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1059&slc_lang=fa&sid=1 Alireza Kazemizadeh علیرضا کاظمی‌زاده alirezakazemizadeh@yahoo.com 5200319475328460051422 5200319475328460051422 Yes Dept. of Chemistry, Faculty of Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran دانشگاه آزاد اسلامی واحد زنجان، زنجان Mojtaba Salouti مجتبی صلوتی 5200319475328460051423 5200319475328460051423 No Biology Research Center, Islamic Azad University, Zanjan Branch, Zanjan, Iran دانشگاه آزاد اسلامی واحد زنجان، زنجان Azam Ahangari اعظم آهنگری 5200319475328460051424 5200319475328460051424 No Young Researchers and Elite Club, Islamic Azad University, Hidaj Branch, Hidaj, Iran دانشگاه آزاد اسلامی واحد هیدج، هیدج Nahid Shajari ناهید شجری 5200319475328460051425 5200319475328460051425 No Dept. of Chemistry, Faculty of Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran دانشگاه آزاد اسلامی واحد زنجان، زنجان Masoumeh Aghayari معصومه آقایاری 5200319475328460051426 5200319475328460051426 No Young Researchers and Elite Club, Islamic Azad University, Hidaj Branch, Hidaj, Iran دانشگاه آزاد اسلامی واحد هیدج، هیدج Lili Safari Zanjani لیلا صفری زنجانی 5200319475328460051427 5200319475328460051427 No Dept. of Microbiology, Faculty of Sciences, Urmia Branch, Islamic Azad University, Urmia, Iran دانشگاه آزاد اسلامی واحد ارومیه، ارومیه