fa اثر عصاره‌ی هیدروالکلی برگ گیاه غافث (Agrimonia eupatoria L.) بر سمیت کبدی در موش‌های صحرایی نر القا شده با تتراکلرید کربن کارآزمایی بالینی Clinical Trials <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong><em><span style="color:#008080;">Background and Objective:</span> </em></strong>Carbon tetrachloride (CCl₄) is a toxic agent leading to hepatotoxicity. The aim of this study was to investigate the effect of <em>Agrimonia eupatoria</em> leaf hydroalcoholic extract (AEE) on carbon tetrachloride induced rat liver.<br> <span style="color:#008080;"><strong><em>Materials and Methods:</em></strong></span> In this study, 42 male rats were randomly allocated to 6 groups: the control group receiving normal saline, 2ml/kg, ip and the sham group receiving olive oil, 2ml/kg, ip, the CCl_4&nbsp;&nbsp;induced group receiving carbon tetrachloride/olive oil&nbsp; (1:1), 2ml/kg, single dose, ip. The treated groups (1, 2 and 3) were induced by carbon tetrachloride 1:1 with olive oil, 2ml/kg, single dose, ip and after two hours underwent treatment by 200 mg/kg, 400 mg/kg and 800 mg/kg AEE/day for 96 hrs, ip. Then, the blood samples were collected from their heart directly and subsequently serum levels of the liver enzymes were measured. The microscopic studies of liver tissue were performed. The data were analyzed with ANOVA and Tukey tests.<br> <strong><em><span style="color:#008080;">Results:</span> </em></strong>The results of this study showed that the serum levels of liver enzymes significantly increased in the group receiving CCl₄ compared to the control group. Meanwhile, treatment by AEE significantly decreased the serum levels of liver enzymes in treated groups compared to the group receiving CCl₄ (P<0.001). Histological study revealed necrosis and inflammation in the liver tissue in CCl₄ group and reduction in necrotic cells and inflammation in AEE treated group.<br> <strong><em><span style="color:#008080;">Conclusion:</span> </em></strong>Carbon tetrachloride can bring about necrosis and inflammation effects on the liver tissue. AEE can decrease toxic effects of carbon tetrachloride in the liver tissue by reducing the serum levels of liver enzymes.&nbsp;&nbsp; </span></span></p> Agrimonia eupatoria L., liver toxicity, Carbon tetrachloride, liver enzymes, Rats 84 97 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1061&slc_lang=fa&sid=1 Maryam Khazaei مریم خزائی maryam.khazaei133@yahoo.com 5200319475328460051440 5200319475328460051440 Yes Dept. of Biology, Bu-Ali Sina University, Hamedan, Iran دانشکده‌ی علوم پایه، دانشگاه بوعلی سینا، همدان Naser Mirazi ناصر میرازی 5200319475328460051441 5200319475328460051441 No Dept. of Biology, Bu-Ali Sina University, Hamedan, Iran دانشکده‌ی علوم پایه، دانشگاه بوعلی سینا، همدان