fa نقش پمپ تراوشی AcrAB در مقاومت به فلوروکینولون‌ها در جدایه‌های کلبسیلا پنومونیه جمع آوری شده از مراکز پزشکی کرمانشاه کارآزمایی بالینی Clinical Trials <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#008080;"><strong><em>Background and Objective</em></strong>:</span> One of the mechanisms involved in the resistance of Klebsiella pneumoniae to fluoroquinolones is AcrAB leakage pumps.&nbsp; In this study, the prevalence of Klebsiella pneumoniae isolates with AcrAB encoding genes and the role of permeation pumps in fluoroquinolone resistance were investigated.<br> <span style="color:#008080;"><strong><em>Materials and Methods</em></strong>:</span> 165 samples from patients of Kermanshah Medical Centers were collected and 100 isolates of Klebsiella pneumoniae approved by API-20E kit. Antibiotic susceptibility of isolates to ciprofloxacin and levofloxacin was determined using disk diffusion and MIC (Minimum Inhibitory Concentration) experiments. AcrA and AcrB genes were determined by PCR method. The effect of bacterial permeation inhibitor (CCCP) Carbonyl cyanide m-chlorophynyl hydrazone with final concentration of 5&mu;g / ml on MIC of fluoroquinolones resistant isolates was done using agar dilution method.<br> <span style="color:#008080;"><strong><em>Results:</em></strong></span> Of the100 Klebsiella pneumoniae isolates, 28 (28%), were resistant to ciprofloxacin and levofloxacin. Both acrA and acrB genes were resistant to antibiotics in all Klebsiella pneumoniae isolates. The effect of CCCP on isolates resistant to ciprofloxacin and levofloxacin resulted in a decrease in MIC in 11 isolates (39.2%), among which the MIC was changed in five isolated from resistant to susceptible state.<br> <span style="color:#008080;"><strong><em>Conclusion:</em></strong></span> Although the high drug resistance of isolates is not due to permeation pumps, the results of inhibition of pump indicate their role in the resistance of 39% of isolates. Also, the presence of AcrAB genes in all fluoroquinolones resistant Klebsiella pneumoniae isolates shows the importance of these pumps.</span></span></p> Keywords: AcrAB, leakage pump, Fluoroquinolone, Klebsiella pneumonia 12 21 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1068&slc_lang=fa&sid=1 Alisha Akya علی شا اکیا 5200319475328460051225 5200319475328460051225 No Nosocomial Infection Research Centre, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran دکترای تخصصی باکتری شناسی، دانشیار مرکز تحقیقات عفونت‌های بیمارستانی، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه Azam Elahi اعظم الهی azamelahi1365@gmail.com 5200319475328460051226 5200319475328460051226 Yes Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran کارشناسی ارشد میکروب‌شناسی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه Roya Chegeneh Lorestani رویا چگنه لرستانی 5200319475328460051227 5200319475328460051227 No Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran کارشناسی ارشد میکروب‌شناسی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه Keyghobad Ghadiri کیقباد قدیری 5200319475328460051228 5200319475328460051228 No Nosocomial Infection Research Centre, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran فوق تخصص بیماری‌های عفونی کودکان، دانشیار مرکز تحقیقات عفونت‌های بیمارستانی، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه