fa ارزیابی وجود جهش‌زایی مستقیم در چند نوع از رنگ‌های طبیعی خوراکی مورد استفاده در مواد غذایی با آزمون Ames کارآزمایی بالینی Clinical Trials <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#008080;"><strong><em>Background and Objective</em></strong>:</span> Nowadays, colors are widely used in various food industries, including sweets, confectionery, ice cream, beverages, and so on. Colors can have asthma-like complications, hyperactivity in children and cancers. Therefore, the aim of this study was to evaluate the direct mutagenic effect of several types of natural colors&nbsp;via&nbsp;Ames method.<br> <span style="color:#008080;"><strong><em>Materials and Methods</em></strong>:</span> After preparation of 6 natural colors from three different brands, their direct mutagenesis was investigated by Ames test. In this test, the strains of Salmonella typhimurium TA98 and TA100, carrying the selective mutation in His-Hiscoprotein Histidine, were cultured on glucose culture medium at least in the presence of color samples, and only the bacteria that had His + mutation were colonized.<br> <span style="color:#008080;"><strong><em>Results</em></strong>:</span> Direct mutagenicity of the food colors was measured by counting colonies in the plates containing edible color and comparing it with negative controls. The results showed that the number of referent colonies in plates containing cherry red&nbsp;and golden yellow for company B for both strains and the dark brown color of company B and C, and orange color for TA100 and TA98 strains in some concentrations were more than twice comparing the negative control plates and had direct mutagenic effects. The dark green and light green colors of the A, B, C, and golden yellow for companies A, C and cherry red&nbsp;and orange for&nbsp;companies C did not show direct mutagenic&nbsp;activity.<br> <span style="color:#008080;"><strong><em>Conclusion</em></strong><em>:</em></span> Based on these results, some of the studied colors, despite being declared natural, showed direct mutagenic activity.</span></span></p> Direct mutation, Natural edible color, Ames test, TA100, TA98 53 68 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1072&slc_lang=fa&sid=1 Haleh AkBari هاله اکبری 5200319475328460051376 5200319475328460051376 No Dept.of Food Safety and Hygiene, Faculty of Paramedical, Zanjan University of Medical Sciences, Zanjan, Iran کارشناسی ارشد بهداشت و ایمنی مواد غذایی، دانشکده بهداشت و پیراپزشکی، دانشگاه علوم پزشکی زنجان، زنجان Habib Zeighami حبیب ضیغمی 5200319475328460051377 5200319475328460051377 No Dept. of Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran دکترای تخصصی باکتری شناسی، استادیار گروه میکروب شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان، زنجان Mehran Mohseni مهران محسنی mohsenim@zums.ac.ir 5200319475328460051378 5200319475328460051378 Yes Dept. of Food and Drug control, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran دکترای تخصصی مواد خوراکی، دانشیار گروه کنترل غذا و دارو، دانشکده داروسازی، دانشگاه علوم پزشکی زنجان، زنجان