fa بررسی بیان ژن کاسپاز 9 و القای آپوپتوز پس از فوتودینامیک تراپی با روی فتالوسیانین در رده‌ی سلولیSW872 سرطان پوست کارآزمایی بالینی Clinical Trials <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#008080;"><strong><em>Background and Objective</em></strong>:</span> The treatment of cancer comes as a great challenge worldwide. Thus the development of effective therapies with minimal side effects is important. Photodynamic therapy is a non-invasive and new therapeutic approach for the treatment of cancer. Therefore, in this study, we evaluated the photodynamic effects of a light-sensitive compound, Zinc-phthalocyanine (ZnPc), on cell growth and the expression of caspase-9 in SW872 cell line<span dir="RTL">.</span><br> <strong><em><span style="color:#008080;">Materials and Methods:</span> </em></strong>For cytotoxic effects of photodynamic therapy with ZnPc, MTT assay was conducted. Quantifying the level of caspase-9 gene expression was carried out with qRT-PCR. The statistical analysis was done by GraphPad Prism 6 software<span dir="RTL">.</span><br> <span style="color:#008080;"><strong><em>Results:</em></strong></span> MTT assay results showed that photodynamic therapy with ZnPc significantly reduced cancer cell growth. The IC50 for cell line SW872 was 0/034 &mu;g/ml in a 24 J/cm2 light source. qRT-PCR results showed that photodynamic therapy with ZnPc increased the gene expression of caspase-9 in the treated group<span dir="RTL">.</span><br> <span style="color:#008080;"><strong><em>Conclusion:</em></strong></span> Photodynamic therapy with ZnPc resulted in decreased cell growth and induced apoptosis in a concentration-dependent manner in SW872 cell lines. Based on the results, photodynamic therapy with ZnPc can be a powerful therapeutic approach for the treatment of skin cancer with minimal side effect<strong>s</strong>.</span></span></p> Key Words: Photodynamic therapy, Caspase 9, Apoptosis, SW872 9 19 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1080&slc_lang=fa&sid=1 Mohammad Amin Doustvandi محمدامین دوستوندی m.mashayekhi@iaut.ac.ir 5200319475328460051007 5200319475328460051007 Yes Dept. of Genetics, Islamic Azad University, Tabriz Branch, Tabriz, Iran دانشگاه آزاد اسلامی واحد تبریز، تبریز Fatemeh Mohammadnejad فاطمه محمدنژاد 5200319475328460051008 5200319475328460051008 No Dept. of Genetics, Islamic Azad University, Tabriz Branch, Tabriz, Iran دانشگاه آزاد اسلامی واحد تبریز، تبریز MMohammad Reza Mashayekhi محمد‌رضا مشایخی 5200319475328460051009 5200319475328460051009 No Dept. of Genetics, Islamic Azad University, Tabriz Branch, Tabriz, Iran دانشگاه آزاد اسلامی واحد تبریز، تبریز