fa تاثیر دوکوزاهگزانوئیک اسید بر یادگیری و حافظه فضایی در مدل بیماری آلزایمر موش‌های صحرایی نر بالغ کارآزمایی بالینی Clinical Trials <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#008080;"><strong><em>Background and Objective:</em></strong></span> Docosahexaenoic acid (DHA) is the major polyunsaturated fatty acid in the brain and a structural component of neuronal membranes. The present study aimed to examine the effect of DHA on spatial learning and memory in an electrical obliteration of nucleus basalis magnocellularis (NBM) model of Alzheimer&#39;s disease.<br> <span style="color:#008080;"><strong><em>Materials and Methods:</em></strong></span> In this study 56 adult male Wistar rats were divided into eight groups: control, lesion (which received bilateral electrical lesions of the NBM), sham (the electrode was entered into the NBM with no lesion), lesion + DHA (0.1, 1, 2.5 and 5 mg/kg; ip) and Vehicle (lesion + Sesame Oil 0.2mL). After one week, the rats were trained to perform the Y-maze task for five days. Twenty-five days after training, a retention test was performed to determine long-term memory.<br> <span style="color:#008080;"><strong><em>Results:</em></strong></span> Bilateral NBM lesion reduced spatial learning in comparison with control and sham groups (P<0.001). No effect on spatial learning was seen in the vehicle group compared with the lesion group. Treatment with DHA in the following groups; lesion + DHA 1mg/kg (P<0.05), lesion + DHA 2.5mg/kg (P<0.01), lesion + DHA 5mg/kg (P<0.01), significantly improved spatial learning. Moreover, no significant difference was observed between the results on the 5th day of training and memory retention on the 30th day.<br> <span style="color:#008080;"><strong><em>Conclusion:</em></strong></span> From the results of this study, it can be concluded that Docosahexaenoic acid improves spatial learning defects in an electrical lesion of NBM model of Alzheimer&#39;s disease in a dose-dependent manner.</span></span></p> Keywords: Docosahexaenoic acid, Spatial learning, Nucleus basalis of magnocellularis, Alzheimer’s disease 48 60 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1082&slc_lang=fa&sid=1 Nastaran Zamani نسترن زمانی Na_zamani2000@yahoo.com 5200319475328460051019 5200319475328460051019 Yes Dept. of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran. دانشجوی دکتری تخصصی فیزیولوژی جانوری، گروه زیست شناسی، دانشکده‌ی علوم، دانشگاه شهید چمران اهواز، اهواز Ahmad Ali Moazedi احمدعلی معاضدی 5200319475328460051020 5200319475328460051020 No Dept. of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran. دکتری تخصصی نوروفیزیولوژی، استاد گروه زیست شناسی، دانشکده‌ی علوم دانشگاه شهید چمران اهواز، اهواز MOhammad Reza Adarinesh Khaki محمدرضا آفرینش خاکی 5200319475328460051021 5200319475328460051021 No Cognitive Research Center, Kerman University of Medical Sciences Sciences, Kerman, Iran. دکتری تخصصی نوروفیزیولوژی، استادیار مرکز تحقیقات علوم شناختی و علوم اعصاب کرمان، دانشگاه علوم پزشکی کرمان، کرمان Mehdi Pourmehdi Boroujeni مهدی پور مهدی بروجنی 5200319475328460051022 5200319475328460051022 No Dept. of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran. دکتری تخصصی اپیدمیولوژی، دانشیار گروه بهداشت و مواد غذایی دانشکده‌ی دامپزشکی دانشگاه شهید چمران اهواز، اهواز