fa مهار رشد و تکثیر سلول‌های سرطانی معده توسط جنیستئین از طریق مطالعه p38MAPK کارآزمایی بالینی Clinical Trials <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#008080;"><strong><em>Background and Objective:</em></strong></span> Compelling evidence exists in favor of the effectiveness of herbal plant-derived components in cancer treatment; however their exact mechanisms of action are not well understood. Since the alteration of mitogen-activated protein kinase enzymes (MAPKs) has been reported in different cancer types, the present study aimed to investigate the effects of soy isoflavonoid genistein on the inhibition of cell viability and proliferation of AGS gastric cancer cell line by determining p38MAPK gene expression and also protein levels.<br> <span style="color:#008080;"><strong><em>Materials and Methods:</em></strong></span> Cell viability was determined by MTT assay at different genistein concentrations (0, 50, 70, and 90 &micro;M) after 24 hours of incubation. Quantitative Real-time PCR was carried out to obtain p38MAPK gene expression levels and its active phosphorylated protein (p-p38MAPK) was measured by flow cytometry.<br> <span style="color:#008080;"><strong><em>Results:</em></strong></span> Genistein significantly reduced cell viability in a concentration and time-dependent manner.&nbsp; Exposure of gastric cancer cells to 0, 50, 70, and 90 &micro;M genistein down-regulated p38MAPK gene expression by 83, 56, and 57%, and reduced cell proliferation by 35, 52, and 67%, respectively.&nbsp; In addition, a great reduction was observed in p-p38MAPK protein levels in treated cells compared to untreated control cells.<br> <span style="color:#008080;"><strong><em>Conclusion:</em></strong></span> Since different concentrations of genistein reduced p38MAPK gene expression and lowered proliferation of AGS gastric cancer cells, it might be a potent candidate for a therapeutic plant-derived agent for combination therapy in gastric cancer.</span></span></p> Keywords: Apoptosis, Gastric neoplasms, Gene expression, Genistein, p38 mitogen-activated protein kinase, Proliferation 88 99 http://journal.zums.ac.ir/browse.php?a_code=A-10-4-1083&slc_lang=fa&sid=1 Iraj Khodadadi ایرج خدادادی khodadadi@umsha.ac.ir 5200319475328460050985 5200319475328460050985 Yes Dept. of Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan-Iran دکترای تخصصی بیوشیمی، دانشیار گروه بیوشیمی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی همدان، همدان Neda Ghasemkhani ندا قاسم‌خانی 5200319475328460050986 5200319475328460050986 No Dept. of Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan-Iran کارشناس ارشد گروه بیوشیمی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی همدان، همدان Gholam Reza Shafiee غلامرضا شفیعی 5200319475328460050987 5200319475328460050987 No Dept. of Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan-Iran دکترای تخصصی بیوشیمی، استادیار گروه بیوشیمی، دانشکده‌ی پزشکی، دانشگاه علوم پزشکی همدان، همدان