en اثر مهاری فلوکونازول همراه با آمفوتریسین B بر بیوفیلم کاندیدا آلبیکنس مقاوم در برابر فلوکونازول Medical Laboratory and Animal Investigation Medical Laboratory and Animal Investigation مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:times new roman;"><span style="color:#008080;"><strong>Background & Objective:</strong></span> The incidence of biofilm-related infections caused by <em>Candida albicans</em> has increased dramatically. <em>C. albicans</em> biofilm-related infections are more resistant to antifungal medications. This work was an attempt to examine inhibitory effects of fluconazole in combination with amphotericin B on fluconazole-resistant <em>C. albicans</em> biofilm.<br> <span style="color:#008080;"><strong>&nbsp;Materials & Methods:</strong></span> Fluconazole-resistant <em>C. albicans</em> was identified among colonized clinical isolates of <em>C.</em> <em>albicans</em> obtained from the patients at Shahid Beheshti Hospital, Yasooj, Iran, between September 2016 and January 2017<em>.</em> The MICs of fluconazole alone and in combination with amphotericin B were determined on fluconazole-resistant<em> C. albicans</em> by broth microdilution method based on the CLSI document M27-A3 and the synergistic effects were interpreted by the FIC index. To address the inhibitory effects of drug combinations, we investigated the anti-biofilm activities of fluconazole alone and in combination with amphotericin B against fluconazole-resistant<em> C. albicans</em> using crystal violet staining, light field microscopy and expression analysis of <em>HWP1</em> using RT-PCR.<br> <span style="color:#008080;"><strong>Results: </strong></span>Ten percent of the colonizing clinical isolates of <em>C. albicans</em> were found to be fluconazole-resistant. The fluconazole combined with amphotericin B exhibited synergistic and partial synergistic effects against fluconazole-resistant<em> C.</em>&nbsp;<em>albicans</em> isolates with FIC index ranging from 0.50 to 0.75. Our findings demonstrated that fluconazole combined with amphotericin B exerted a significant reduction and inhibition on biomass, biofilm formation and the expression levels of <em>HWP1 </em>(<em>P</em><0.05).<br> <span style="color:#008080;"><strong>Conclusion: </strong></span>Fluconazole combined with amphotericin B showed potent inhibitory activity against fluconazole-resistant <em>C. albicans</em> biofilm formation. Other measures are important to determine the effectiveness of the drug combinations against<em> C. albicans</em> biofilm.</span></span></p> Amphotericin B, Antifungal agents, Biofilm, Candida albicans, Fluconazole 1 8 http://journal.zums.ac.ir/browse.php?a_code=A-10-4500-1&slc_lang=en&sid=1 Sara Neamati سارا نعمتی mnlzdh@gmail.com 5200319475328460047769 5200319475328460047769 No Dept. of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran Alireza Khodavandi علیرضا خداوندی alireza_khodavandi@yahoo.com 5200319475328460047770 5200319475328460047770 Yes Dept. of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran Fahimeh Alizadeh فهیمه علیزاده mnalizadeh@yahoo.com 5200319475328460047771 5200319475328460047771 No Dept. of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran