en فعالیت ضد توموری عصاره متانولی فلزی Zataria در رده سلولی لوسمی پرومیلوسیتیک حاد. NB4 Medical Biology Medical Biology مقاله پژوهشی Original Research Article <div style="text-align: justify;"><strong><span style="color:#008080;">Background & Objective:</span></strong><span style="color:#008080;"> </span>&nbsp;<em>Zataria multiflora</em> is a plant that belongs to <em>Laminaceae</em> family. It is traditionally believed to have several therapeutic effects. Acute promyelocytic leukemia is a distinct subtype of acute myeloid leukemia with dominancy of promyelocytes in bone marrow and blood stream. The aim of this study is to investigate the anticancer effects of <em>Z. multiflora</em> extract on acute promyelocytic leukemia cell lines.<br> <strong><span style="color:#008080;">Materials & Methods:</span></strong><span style="color:#008080;"> </span>&nbsp;Viability of NB4 cells was determined by trypane blue test after treatment with 20, 40 and 80&micro;g/mL of <em>Z. multiflora</em> extract for 24 hours. Then, the metabolic activity of cells was determined by MTT assay after 24 hours of treatment with 80 &micro;g/mL <em>Z. multiflora</em>. Finally, Real-time PCR was employed to study the effect of <em>Z. multiflora</em> extract on the expression of hTERT gene.<br> <span style="color:#008080;"><strong>Results: </strong></span>&nbsp;<em>Z. multiflora</em> extract decreased the viability of NB4 cells in a dose-dependent manner. Metabolic activity of NB4 cells significantly decreased following treatment with 80 &micro;g/mL <em>Z. multiflora</em>. Gene expression analysis showed 59%&plusmn;4% decrease in the expression of hTERT gene after treatment with 80 &micro;g/mL of <em>Z. multiflora</em>.<br> <span style="color:#008080;"><strong>Conclusion: </strong></span>&nbsp;<em>Z. multiflora</em> extract significantly decreased the viability and metabolic activity of NB4 cells. It also led to significant downregulation of hTERT gene compared to the control group. Therefore, <em>Z. multiflora</em> methanolic extract potentially has anticancer effect on acute promyelocytic leukemia cells through down regulation of hTERT. Further investigations are needed to explore other mechanisms of actions and the active ingredients.</div> Zataria multiflora, Tumor Suppressor, Leukemia, Acute Promyelocytic Leukaemia, NB4, hTERT gene 43 47 http://journal.zums.ac.ir/browse.php?a_code=A-10-4576-1&slc_lang=en&sid=1 Iman Baluchi ایمان بلوچی imanbaloochi7175@gmail.com 5200319475328460047269 5200319475328460047269 No Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran Hussein Anani حسین عنانی anani.hossein@gmail.com 5200319475328460047270 5200319475328460047270 No Dept. of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran Alireza Farsinejad علیرضا فارسی‌نژاد farsinezhad239@yahoo.com 5200319475328460047271 5200319475328460047271 No Dept. of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran Ahmad Fatemi احمد فاطمی ahmad.fatemi2@gmail.com 5200319475328460047272 5200319475328460047272 No Dept. of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran Roohollah Mirzaee khalilabadi روح الله میرزایی خلیل آبادی khalilabadi60@gmail.com 5200319475328460047273 5200319475328460047273 Yes Dept. of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran