en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:times new roman;"><span style="color:#008080;"><strong>Background </strong><strong>and Objective: </strong></span>Biofilm formation plays a crucial role in wound infections and increases the bacteria resistance to treatment. The present study investigated the relationship between the biofilm formation, ESBL, AmpC, and KPC enzymes in <em>Acinetobacter baumannii</em> isolated from the wound specimens.<br> <span style="color:#008080;"><strong>Materials and Methods:</strong></span> Eighty- nine <em>A. baumannii</em> isolates were collected from wound specimens and were confirmed by different biochemical tests. The biofilm-producing strains were identified using the crystal violet method. The producing strains of KPC, ESBL, and AmpC &beta;-lactamase enzymes were detected through phenotypic tests. Further, the PCR method was employed to identify the ESBL, KPC, and AmpC. The Chi-square test and SPSS 16 were used for data analysis.<br> <span style="color:#008080;"><strong>Results: </strong></span>Among 89 wound isolates, 21 and 68 were collected from male and female patients, respectively. The strains resistant to ciprofloxacin (69.66%) and gentamicin (66.29%) were the most frequent strains while ceftazidime (7.86%) and colistin (1.12%) resistant strains had the lowest frequency. Furthermore, 40 isolates were considered as ESBL-producing enzymes, 33 isolates as AmpC, and 26 isolates as KPC-producing enzymes. In addition, the isolates were categorized as strong biofilms with 20 isolates, moderate biofilms with 19 isolates, and weak biofilm-producing strains with 10 isolates. The distribution of the &beta;-lactamase genes in <em>A. baumannii</em> isolates was <em>bla</em>VEB (34.83%), <em>bla</em>PER (32.58%), <em>bla</em>FOX (29.21%), <em>bla</em>ADC (30.33%), <em>bla</em>IMP (28.08%), and <em>bla</em>KPC (22.47%).<br> <span style="color:#008080;"><strong>Conclusion:</strong></span> Our results demonstrated that isolates with a higher level of antibiotic resistance tended to form stronger biofilms. Likewise, the results showed that the relationship between biofilm formation and antibiotic resistance might be affected by the type of &beta;-lactamase enzyme in wound infection.</span></span></p> Acinetobacter baumannii, β-lactamases, Biofilm, Bacterial Infections, Drug Resistance 34 42 http://journal.zums.ac.ir/browse.php?a_code=A-10-5171-1&slc_lang=en&sid=1 Mahyar Porbaran mahyarporbaran@gmail.com 5200319475328460048835 5200319475328460048835 No Department of Microbiology, Faculty of Basic Sciences, Hamadan Branch, Islamic Azad University, Hamadan, Iran Reza Habibipour habibipour.r@gmail.com 5200319475328460048836 5200319475328460048836 Yes Department of Microbiology, Faculty of Basic Sciences, Hamadan Branch, Islamic Azad University, Hamadan, Iran