Journal of Advances in Medical and Biomedical Research

en Cerasus microcarpa and Amygdalus scoparia Methanolic Extract Protect Cultured Cerebellar Granule Neurons Against β-amyloid-induced Toxicity and Oxidative Stress Pharmacology Pharmacology مقاله پژوهشی Original Research Article <div style="text-align: justify;"> Background & Objective:  Beta-amyloid peptide (Aβ) causes neural cell death and has a pivotal role in the progression of Alzheimer’s disease (AD). The prevention of Aβ-induced toxicity is a target for agents intend to treat Alzheimer’s disease. Our previous in vitro study indicated anti-cholinesterase and anti-oxidant activity of Amygdalus scoparia and Cerasus microcarpa methanolic extracts. In the present study, their neuroprotective effects against Aβ-induced toxicity are investigated.  Materials & Methods:  The methanolic extracts of the aerial parts of A. scoparia and C. microcarpa were prepared by the maceration method. In the culture, mature cerebellar granule neurons (CGNs) were exposed to Aβ alone or in combination with different concentrations of extracts and incubated for 24 hours, and cell viability was measured by the MTT assay. Oxidative stress markers and AChE activity were also measured. Then, the AChE activity of cultured neurons was measured after incubation with different concentrations of extracts. The LD50 values of extracts were estimated using the limit test. Results:  The co-incubation of C. microcarpa and A. scoparia extracts with Aβ protected CGNs against Aβ-induced cell death and ameliorated Aβ-induced oxidative stress. The AChE activity of cultured neurons was inhibited by both extracts in a dose-dependent manner. LD50 was estimated as being above 2000 mg/kg for both extracts. Conclusion:  Both extracts attenuated Aβ-induced cell death by ameliorating oxidative stress. Also, the inhibitory effect of extracts on AChE activity might have been involved. Based on these results, these extracts may have therapeutic effects on Alzheimer’s disease.  However, further investigations are recommended.</div> Alzheimer, Oxidative stress, Acetylcholinesterase, Cerasus microcarpa, Amygdalus scoparia 23 32 http://journal.zums.ac.ir/browse.php?a_code=A-10-5211-1&slc_lang=en&sid=1 Nasim Ataei nassim_ataeii@yahoo.com 5200319475328460049435 5200319475328460049435 No Dept. of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Maliheh Soodi soodi@modares.ac.ir 5200319475328460049436 5200319475328460049436 Yes Dept. of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Homa Hajimehdipoor hajimehd@sbmu.ac.ir 5200319475328460049437 5200319475328460049437 No Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Sholeh Akbari sholehakbari91@gmail.com 5200319475328460049438 5200319475328460049438 No Dept. of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Mahshid Alimohammadi alimohamadi.mahshid@gmail.com 5200319475328460049439 5200319475328460049439 No Dept. of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran