en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><span style="color:#ffffff;"><strong><span style="background-color:#008080;">Background and Objective</span></strong><span style="background-color:#008080;">:</span></span> Spermatogenesis and oogenesis stress is a primary epigenetic element that can cause lifelong modifications in neural system functions. This study was designed to elucidate whether preconceptional stress during spermatogenesis and oogenesis affects the seizure behaviors and body weight of the offspring later in life.<br> <span style="color:#ffffff;"><strong><span style="background-color:#008080;">Materials and Methods</span></strong><span style="background-color:#008080;">:</span></span> In this experimental study, 16 male and 32 female adult rats were included. Half of the rats were subjected to predatory stress. Then, they were coupled as follows: both subjects were intact as the control group (MC-FC), both subjects were stressed (MS-FS), male control/female stressed (MC-FS), and male stressed/female control (MS-FC). The pups were weighed and assessed for pentylenetetrazole-induced seizures. In addition, aniline blue staining was performed to study the chromatin maturity of the sperm in male parents immediately after copulation.<br> <span style="color:#ffffff;"><strong><span style="background-color:#008080;">Results:</span></strong></span> The latency of the first seizure behavior significantly diminished in stressed pups, and the duration of focal and generalized seizures significantly increased in the stressed rats<span dir="RTL">.</span> In addition, the body weight of the pups decreased in the stress groups compared to control rats, and a significant decrease was detected in the chromatin maturity of sperms in stressed rats.<br> <span style="color:#ffffff;"><strong><span style="background-color:#008080;">Conclusion:</span></strong></span> Stress during spermatogenesis and oogenesis can be critical for the general growth (body weight) and seizure susceptibility of the offspring. Therefore, to improve reproductive outcomes, stress-lowering interventions are better to be started before conception.</span></span></p> Body weight, Preconceptional , Stress disorder, Rats, Seizures 97 104 http://journal.zums.ac.ir/browse.php?a_code=A-10-5294-1&slc_lang=en&sid=1 Ehsan Saboory saboory@zums.ac.ir 5200319475328460051685 5200319475328460051685 Yes Zanjan Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran Maryam Mahmoodkani maryam.mahmodkhani@gmail.com 5200319475328460051686 5200319475328460051686 No Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran Shiva Roshan-Milani shiva_muk@yahoo.com 5200319475328460051687 5200319475328460051687 No Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran Yousef Rasmi yrasmi@gmail.com 5200319475328460051688 5200319475328460051688 No Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran