en Medical Biology Medical Biology مقاله پژوهشی Original Research Article <p style="text-align: justify;"><span style="font-size:16px;"><span style="font-family:Times New Roman;"><strong><span style="color:#ffffff;"><span style="background-color:#008080;">Background and Objective:</span></span> </strong>The mechanism of analgesic and anxiolytic activity of resveratrol in neuropathic pain conditions remains obscure. The present study was conducted to examine whether the analgesic and anxiolytic activities of resveratrol are associated with &alpha;₁- and &alpha;₂-adrenoceptors of the anterior cingulate cortex (ACC), which is a key area of the cortex in the pain process, following neuropathic pain in rats.<br> <strong><span style="color:#ffffff;"><span style="background-color:#008080;">Materials and Methods:</span></span> </strong>Neuropathic pain was created by chronic constriction injury (CCI) of the sciatic nerve. Male Wistar rats were assigned to the sham, CCI, CCI+resveratrol (40&mu;g/5&mu;L), CCI+resveratrol+prazosin (&alpha;₁-adrenoceptor antagonist,30&mu;g/5&mu;L), and CCI+resveratrol-Yohimbine (&alpha;₂-adrenoceptor antagonist, 30&mu;g/5&mu;L) groups. The rats received intra-ACC injection of the drug on the day of CCI and for 6 days post-CCI on a daily basis. Cold allodynia (using acetone test) and anxiety (using elevated plus maze, EPM) were examined on days 2, 4, and 6 following CCI.<br> <strong><span style="color:#ffffff;"><span style="background-color:#008080;">Results:</span></span> </strong>CCI model significantly increased cold allodynia and anxiety. Resveratrol significantly decreased cold allodynia. Prazosin induced no significant changes in allodynia as compared with the CCI+resveratrol treated group. But the animals in this group had no significant difference from the day before the surgery or compared with the sham group. Prazosin significantly decreased entries into open arms. Additionally, yohimbine significantly increased cold allodynia as compared with the CCI+resveratrol treated group. However, it induced no significant changes in the EPM parameters. Our findings also demonstrated a significant correlation between allodynia and anxiety in CCI rats.<br> <strong><span style="color:#ffffff;"><span style="background-color:#008080;">Conclusion:</span></span> </strong>It is suggested that the mechanism of analgesic and anxiolytic activities of resveratrol in the ACC of rats is different, and is mediated through &alpha;₂- and &alpha;₁-adrenoceptors, respectively.</span></span></p> Allodynia, Anxiety, Anterior cingulate cortex, α-adrenoceptors, Rat, Resveratrol 183 190 http://journal.zums.ac.ir/browse.php?a_code=A-10-5228-2&slc_lang=en&sid=1 Mohammad Hassan Mirasheh mohammadmirasheh30@gmail.com 5200319475328460052538 5200319475328460052538 No Students Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran Mohammad Reza Zohrehvand zohrevand.mr1@gmail.com 5200319475328460052539 5200319475328460052539 No Students Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran Reza Kazemi reza76kazemi@gmail.com 5200319475328460052540 5200319475328460052540 No Students Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran Zahra Bahari bahari_441@yahoo.com 5200319475328460052541 5200319475328460052541 Yes Dept. of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Farideh Bahrami farideh_bahrami@yahoo.com 5200319475328460052542 5200319475328460052542 No Dept. of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Zohreh Jangravi zohrehjangravi@yahoo.com 5200319475328460052543 5200319475328460052543 No Dept. of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Mehdi Graily mehdigraily@gmail.com 5200319475328460052544 5200319475328460052544 No Dept. of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran